Laboratory Experiments in Microbiology (11th Edition)
Laboratory Experiments in Microbiology (11th Edition)
11th Edition
ISBN: 9780321994936
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 13, Problem 1Q
Summary Introduction

To identify:

The substance that cannot be found in the blue area of the starch agar plate, but in the clear area.

Introduction:

The starch is a polysaccharide made up of glucose. The glucose is linked by glycosidic bonds, which can be hydrolyzed using the amylase enzyme.

Expert Solution & Answer
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Explanation of Solution

The iodine test is the used to test the presence of starch. The starch turns the aqueous solution of triiodide into an intense blue-black color. This is due to the formation of intermolecular charge transfer complex.

The digestion of the starch by amylase gives a negative iodine test, as the amylase converts the starch to glucose. The clear region on a starch agar plate gives a negative test due to the absence of starch and its digestion into glucose.

Conclusion

The glucose and amylase can be found in the clear area.

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