The reason for the preference of sticky ends over blunt ends generated by restriction endonucleases. Introduction: Restriction enzymes are also known as molecular scissors. These cut the DNA fragments at recognition sites and produce restriction fragments. Restriction enzymes or endonucleases were discovered by Arber, Nathans , and Smith . Some of the examples of restriction enzymes are Hind III, EcoRI, BamHI, HpaI, and SmaI.

Question

Want to see more full solutions like this?

Answer 1

Question

Chapter 12.4, Problem 11SAQ

Summary Introduction

To explain:

The reason for the preference of sticky ends over blunt ends generated by restriction endonucleases.

Introduction:

Restriction enzymes are also known as molecular scissors. These cut the DNA fragments at recognition sites and produce restriction fragments. Restriction enzymes or endonucleases were discovered by Arber, Nathans, and Smith. Some of the examples of restriction enzymes are Hind III, EcoRI, BamHI, HpaI, and SmaI.

Expert Solution & Answer

Explanation of Solution

Pictorial representation:

Biology: How Life Works, Chapter 12.4, Problem 11SAQ

The restriction fragments produced by restriction enzymes can either have blunt ends or sticky ends. Sticky ends generated by restriction enzymes like EcoRI, HindIII, and BamHI are of great value as they can be attached easily with other sticky ends. The sticky end produced by one restriction enzyme can only attach with the sticky end produced by that restriction enzyme only. However, blunt end of one restriction enzyme can attach to any other blunt end.

Conclusion

Sticky ends produced by one restriction enzyme are preferred because they can easily ligate with other fragments of the DNA.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Students have asked these similar questions

Give only the mode of inheritance consistent with all three pedigrees and only two reasons that support this, nothing more, (it shouldn't take too long)

O

Describe the principle of homeostasis.

Answer 2

Question

Chapter 12.4, Problem 11SAQ

Summary Introduction

To explain:

The reason for the preference of sticky ends over blunt ends generated by restriction endonucleases.

Introduction:

Restriction enzymes are also known as molecular scissors. These cut the DNA fragments at recognition sites and produce restriction fragments. Restriction enzymes or endonucleases were discovered by Arber, Nathans, and Smith. Some of the examples of restriction enzymes are Hind III, EcoRI, BamHI, HpaI, and SmaI.

Expert Solution & Answer

Explanation of Solution

Pictorial representation:

Biology: How Life Works, Chapter 12.4, Problem 11SAQ

The restriction fragments produced by restriction enzymes can either have blunt ends or sticky ends. Sticky ends generated by restriction enzymes like EcoRI, HindIII, and BamHI are of great value as they can be attached easily with other sticky ends. The sticky end produced by one restriction enzyme can only attach with the sticky end produced by that restriction enzyme only. However, blunt end of one restriction enzyme can attach to any other blunt end.

Conclusion

Sticky ends produced by one restriction enzyme are preferred because they can easily ligate with other fragments of the DNA.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 3

Question

Chapter 12.4, Problem 11SAQ

Summary Introduction

To explain:

The reason for the preference of sticky ends over blunt ends generated by restriction endonucleases.

Introduction:

Restriction enzymes are also known as molecular scissors. These cut the DNA fragments at recognition sites and produce restriction fragments. Restriction enzymes or endonucleases were discovered by Arber, Nathans, and Smith. Some of the examples of restriction enzymes are Hind III, EcoRI, BamHI, HpaI, and SmaI.

Expert Solution & Answer

Explanation of Solution

Pictorial representation:

Biology: How Life Works, Chapter 12.4, Problem 11SAQ

The restriction fragments produced by restriction enzymes can either have blunt ends or sticky ends. Sticky ends generated by restriction enzymes like EcoRI, HindIII, and BamHI are of great value as they can be attached easily with other sticky ends. The sticky end produced by one restriction enzyme can only attach with the sticky end produced by that restriction enzyme only. However, blunt end of one restriction enzyme can attach to any other blunt end.

Conclusion

Sticky ends produced by one restriction enzyme are preferred because they can easily ligate with other fragments of the DNA.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 4

Question

Chapter 12.4, Problem 11SAQ

Summary Introduction

To explain:

The reason for the preference of sticky ends over blunt ends generated by restriction endonucleases.

Introduction:

Restriction enzymes are also known as molecular scissors. These cut the DNA fragments at recognition sites and produce restriction fragments. Restriction enzymes or endonucleases were discovered by Arber, Nathans, and Smith. Some of the examples of restriction enzymes are Hind III, EcoRI, BamHI, HpaI, and SmaI.

Expert Solution & Answer

Explanation of Solution

Pictorial representation:

Biology: How Life Works, Chapter 12.4, Problem 11SAQ

The restriction fragments produced by restriction enzymes can either have blunt ends or sticky ends. Sticky ends generated by restriction enzymes like EcoRI, HindIII, and BamHI are of great value as they can be attached easily with other sticky ends. The sticky end produced by one restriction enzyme can only attach with the sticky end produced by that restriction enzyme only. However, blunt end of one restriction enzyme can attach to any other blunt end.

Conclusion

Sticky ends produced by one restriction enzyme are preferred because they can easily ligate with other fragments of the DNA.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 5

Chapter 12.4, Problem 11SAQ

Summary Introduction

To explain:

The reason for the preference of sticky ends over blunt ends generated by restriction endonucleases.

Introduction:

Restriction enzymes are also known as molecular scissors. These cut the DNA fragments at recognition sites and produce restriction fragments. Restriction enzymes or endonucleases were discovered by Arber, Nathans, and Smith. Some of the examples of restriction enzymes are Hind III, EcoRI, BamHI, HpaI, and SmaI.

Expert Solution & Answer

Explanation of Solution

Pictorial representation:

Biology: How Life Works, Chapter 12.4, Problem 11SAQ

The restriction fragments produced by restriction enzymes can either have blunt ends or sticky ends. Sticky ends generated by restriction enzymes like EcoRI, HindIII, and BamHI are of great value as they can be attached easily with other sticky ends. The sticky end produced by one restriction enzyme can only attach with the sticky end produced by that restriction enzyme only. However, blunt end of one restriction enzyme can attach to any other blunt end.

Conclusion

Sticky ends produced by one restriction enzyme are preferred because they can easily ligate with other fragments of the DNA.

Answer 6

Chapter 12.4, Problem 11SAQ

Summary Introduction

To explain:

The reason for the preference of sticky ends over blunt ends generated by restriction endonucleases.

Introduction:

Restriction enzymes are also known as molecular scissors. These cut the DNA fragments at recognition sites and produce restriction fragments. Restriction enzymes or endonucleases were discovered by Arber, Nathans, and Smith. Some of the examples of restriction enzymes are Hind III, EcoRI, BamHI, HpaI, and SmaI.

Expert Solution & Answer

Explanation of Solution

Pictorial representation:

Biology: How Life Works, Chapter 12.4, Problem 11SAQ

The restriction fragments produced by restriction enzymes can either have blunt ends or sticky ends. Sticky ends generated by restriction enzymes like EcoRI, HindIII, and BamHI are of great value as they can be attached easily with other sticky ends. The sticky end produced by one restriction enzyme can only attach with the sticky end produced by that restriction enzyme only. However, blunt end of one restriction enzyme can attach to any other blunt end.

Conclusion

Sticky ends produced by one restriction enzyme are preferred because they can easily ligate with other fragments of the DNA.

Answer 7

Chapter 12.4, Problem 11SAQ

Summary Introduction

To explain:

The reason for the preference of sticky ends over blunt ends generated by restriction endonucleases.

Introduction:

Restriction enzymes are also known as molecular scissors. These cut the DNA fragments at recognition sites and produce restriction fragments. Restriction enzymes or endonucleases were discovered by Arber, Nathans, and Smith. Some of the examples of restriction enzymes are Hind III, EcoRI, BamHI, HpaI, and SmaI.

Answer 8

Expert Solution & Answer

Explanation of Solution

Pictorial representation:

Biology: How Life Works, Chapter 12.4, Problem 11SAQ

The restriction fragments produced by restriction enzymes can either have blunt ends or sticky ends. Sticky ends generated by restriction enzymes like EcoRI, HindIII, and BamHI are of great value as they can be attached easily with other sticky ends. The sticky end produced by one restriction enzyme can only attach with the sticky end produced by that restriction enzyme only. However, blunt end of one restriction enzyme can attach to any other blunt end.