Chapter 12, Problem 11E

Summary Introduction

To calculate: K_M and V_max values from the given data.

Concept introduction: Enzymes are biological protein catalysts which alter the speed of the reaction in the biological system. The chemical reactions which are catalysed by these enzymes are studied under the enzyme kinetics. The reaction rate is measured based on the rate at which the substrate of the enzymes combines with the enzyme and forms the products. K_M is the Michaelis-Menton constant. K_M value that is not a direct measure of the affinity of the enzyme towards the substrate but concentration of S where the velocity at maximum is half. V_max is the maximum velocity with which the enzyme catalyses the reaction.

$\text{The rate of enzyme reaction }\left(\text{V}\right)\text{ =}\frac{{\text{V}}_{\text{max}}\left[\text{S}\right]}{\left({\text{K}}_{\text{M}}\text{+}\left[\text{S}\right]\right)}$