EP MICROBIOLOGY:W/DISEASES BY..-MOD.ACC
5th Edition
ISBN: 9780134607894
Author: BAUMAN
Publisher: PEARSON CO
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Chapter 11, Problem 7SA
Summary Introduction
To answer:
In what way, the Agrobacterium is used in recombinant DNA technology.
Introduction:
Recombinant DNA technology plays an important role in the field of industry, science, agriculture, and medicine. They generate new genetic combinations by joining two different DNAs (species).
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Describe how would you determine the following using a real-time PCR instrument.
A) The quantity and quality of a DNA sample.
B) Whether an individual had cystic fibrosis from a genomic DNA sample.
C) If several bacterial pathogens were present in DNA extracted from a food sample.
Why are bacteria excellent hosts for recombinant DNA experiments?
Why is it important to use a hyperthermophilic DNA polymerase in PCR?
a) Because only hyperthermophiles have DNA polymerases.
b) Because hyperthermophilic DNA polymerase is able to resist the saline reaction conditions.
c) Because hyperthermophilic DNA polymerase is faster than other polymerases.
d) Because hyperthermophilic DNA polymerase is able to resist denaturation at 95℃.
Chapter 11 Solutions
EP MICROBIOLOGY:W/DISEASES BY..-MOD.ACC
Ch. 11 - Prob. 1TMWCh. 11 - Why are taxonomic names and categories in our...Ch. 11 - Why is it that archaea dont seem to cause disease...Ch. 11 - Prob. 1EDCSCh. 11 - Why are bacteria all classified in the same domain...Ch. 11 - Modified True/False 1. ____ All prokaryotes...Ch. 11 - Prob. 2MTFCh. 11 - Modified True/False 3. __________ If you were to...Ch. 11 - Prob. 4MTFCh. 11 - Prob. 5MTF
Ch. 11 - Modified True/False 6. __________ Halophiles...Ch. 11 - Prob. 7MTFCh. 11 - Prob. 8MTFCh. 11 - Modified True/False 9. A giant bacterium that is...Ch. 11 - Prob. 10MTFCh. 11 - Prob. 1MCCh. 11 - Prob. 2MCCh. 11 - Prob. 3MCCh. 11 - Endospores _____. a. remain alive for decades b....Ch. 11 - Prob. 5MCCh. 11 - Prob. 6MCCh. 11 - Prob. 7MCCh. 11 - Prob. 8MCCh. 11 - Prob. 9MCCh. 11 - The presence of mycolic acid in the cell wall...Ch. 11 - Match the bacterium on the left with the term with...Ch. 11 - Whereas the first edition of Bergeys Manual relied...Ch. 11 - What are extremophiles? Describe two kinds, and...Ch. 11 - Name and describe three types of bacteria...Ch. 11 - Prob. 4SACh. 11 - Prob. 5SACh. 11 - A student was memorizing the arrangements of...Ch. 11 - Prob. 7SACh. 11 - Name and describe five distinct classes of phylum...Ch. 11 - Prob. 9SACh. 11 - Prob. 1VICh. 11 - Prob. 2VICh. 11 - A microbiology student described deeply branching...Ch. 11 - Prob. 2CTCh. 11 - Prob. 3CTCh. 11 - Prob. 4CTCh. 11 - Louis Pasteur said, The role of the infinitely...Ch. 11 - Prob. 6CTCh. 11 - A scientist who discovers a prokaryote living in a...Ch. 11 - Contrast the processes of nitrogen fixation and...Ch. 11 - Prob. 9CTCh. 11 - How do scientists know there are millions of...Ch. 11 - Why might soil-dwelling organisms such as...Ch. 11 - Using the following terms, fill in the following...
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- What are plasmids? What is the importance of plasmids for the recombinant DNA technology?arrow_forwardTransfection efficiency refers to a) total number of transfected cells per dish O b) total number of transfected cells per field of view total amount of plasmid DNA added per sample d) number of cells per 100 that have taken up plasmid DNAarrow_forwardWhy is Agrobacteriummediated genetic engineering transformation inplants considered as natural genetic engineering?arrow_forward
- How are we to know which of the bacteria have taken up the plasmid?arrow_forwardSequence of which of the following cannot be determined using the Maxam Gilbert method?a) Bacteriab) Plantsc) Bacteriophage T7d) Plasmidarrow_forwardWhy do we use an Electron Scanning Microscope to see bacteriophages over other microscopes?arrow_forward
- 1. What is a plasmid? A) An organelle found in plants that conducts photosynthesis. B) Circular extrachromosomal DNA. C) An essential part of the bacterial chromosome. 2. What is the role of the NaOH in plasmid isolation? A) It increases the pH. B) It denatures the DNA. C) Both of the above. 3. In which direction will the DNA migrate in an agarose gel? A) To the positive pole. B) To the negative pole. C) That depends on the sequence of the DNA. 4. What is the function of SDS? A) It denatures proteins. B) It disintegrates the cell membrane. C) Both of the above. 5. To which species group does E. coli belong to? A) Bacteria, B) Fungi. C) Archaea.arrow_forwardWhy can’t Agrobacterium be used for transforming all plants?arrow_forwardA plasmid may A) replicate independently of the chromosome. B) be transferred cell-to-cell during conjugation. C) be integrated into the chromosome. D) replicate independently of the chromosome, integrate into the chromosome, or be transferred cell-to-cell during conjugation.arrow_forward
- Which of the following would be a good application of plasmid transformation? 1) to make copies of DNA 2) to isolate a change in a single nucleotide 3) to separate DNA fragments 4) to sequence DNAarrow_forwardUse the figure of a plasmid below to answer the following questions. 43) Which enzyme was used to produce the molecule in the figure above? A) RNA polymerase C) a restriction enzyme (endonuclease) B) ligase D) DNA polymerasearrow_forwardWhich of the following is not denatured by heating steps during PCR?a) DNA polb) RNA polc) Restriction enzymesd) DNA ligasearrow_forward
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