To examine: Whether the statement “Restriction nucleases cut DNA at specific sites that are always located between genes” is correct or incorrect.

Question

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Answer 1

Question

Chapter 10, Problem 8Q

A.

Summary Introduction

To examine: Whether the statement “Restriction nucleases cut DNA at specific sites that are always located between genes” is correct or incorrect.

B.

Summary Introduction

To examine: Whether the statement “DNA migrates toward the positive electrode during electrophoresis” is correct or incorrect.

C.

Summary Introduction

To examine: Whether the statement “Clones isolated from cDNA libraries contain promoter sequences” is correct or incorrect.

D.

Summary Introduction

To explain: Whether the statement “PCR utilizes a heat stable DNA polymerase because for each amplification step, double-stranded DNA must be heat-denatured” is correct or incorrect.

E.

Summary Introduction

To examine: Whether the statement “Digestion of genomic DNA with Alu I, a restriction enzyme that recognizes a four-nucleotide sequence produces fragments that are all exactly 256 nucleotides in length” is correct or incorrect.

F.

Summary Introduction

To examine: Whether the statement “To make a cDNA library, both a DNA polymerase and a reverse transcriptase must be used” is correct or incorrect.

G.

Summary Introduction

To examine: Whether the statement “DNA fingerprinting by PCR relies on the fact that different individuals have different numbers of repeats in STR regions in their genome” is correct or incorrect.

H.

Summary Introduction

To examine: Whether the statement “It is possible for a coding region of a gene to be present in a genomic library prepared from a particular tissue but to be absent from a cDNA library prepared from the same” is correct or incorrect.

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Question #3: In the KeyGene paper, the authors state that it would be useful if pollen from an apomict would transmit apomixis-inducing genes to the female in the cross (assuming the pollen is viable). Assuming there was just one gene conferring gametophytic obligate apomixis, and that the two parents are inbreds, what would be the consequences of such a cross if: a) The apomixis was a dominant trait? Indicate the genotypes and phenotypes (apomict or non- apomict) of the parents, F1 and F2 generations. Remember to include the expected genotypic and phenotypic ratios (or percentages) in the F1 and F2 generations, and to position the female first (left side) in the parental cross. b) The apomixis was a recessive trait? Indicate the genotypes and phenotypes (apomict or non- apomict) of the parents, F1 and F2 generations. Remember to include the expected genotypic and phenotypic ratios (or percentages) in the F1 and F2 generations, and to position the female first (left side) in the…

Question #5: Assume that two genes are identified that confer gametophytic facultative apomixis in soybean. The genes show independent assortment. Recessive alleles at both loci are required for the facultative apomixis. Facultative apomixis is triggered when the temperature at pollination is above 20 degrees C. At temperatures below 20 degrees C, all reproduction is sexual, independent of genotype. A facultative apomict male, capable of producing viable pollen, was crossed with a sexually reproducing female. Assuming the parents are completely inbred, what are the predicted phenotypic ratios (apomict: non-apomict) for the F1, F2, and DH (F1-derived) generations at each of the following temperatures*: a) 15°C? b) 25°C? *for full credit, show crosses and genotypes where appropriate. Remember to position the female first (left side) in the cross. Type your answer here:

Answer 2

Question

Chapter 10, Problem 8Q

A.

Summary Introduction

To examine: Whether the statement “Restriction nucleases cut DNA at specific sites that are always located between genes” is correct or incorrect.

B.

Summary Introduction

To examine: Whether the statement “DNA migrates toward the positive electrode during electrophoresis” is correct or incorrect.

C.

Summary Introduction

To examine: Whether the statement “Clones isolated from cDNA libraries contain promoter sequences” is correct or incorrect.

D.

Summary Introduction

To explain: Whether the statement “PCR utilizes a heat stable DNA polymerase because for each amplification step, double-stranded DNA must be heat-denatured” is correct or incorrect.

E.

Summary Introduction

To examine: Whether the statement “Digestion of genomic DNA with Alu I, a restriction enzyme that recognizes a four-nucleotide sequence produces fragments that are all exactly 256 nucleotides in length” is correct or incorrect.

F.

Summary Introduction

To examine: Whether the statement “To make a cDNA library, both a DNA polymerase and a reverse transcriptase must be used” is correct or incorrect.

G.

Summary Introduction

To examine: Whether the statement “DNA fingerprinting by PCR relies on the fact that different individuals have different numbers of repeats in STR regions in their genome” is correct or incorrect.

H.

Summary Introduction

To examine: Whether the statement “It is possible for a coding region of a gene to be present in a genomic library prepared from a particular tissue but to be absent from a cDNA library prepared from the same” is correct or incorrect.

Expert Solution & Answer

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Answer 3

Question

Chapter 10, Problem 8Q

A.

Summary Introduction

To examine: Whether the statement “Restriction nucleases cut DNA at specific sites that are always located between genes” is correct or incorrect.

B.

Summary Introduction

To examine: Whether the statement “DNA migrates toward the positive electrode during electrophoresis” is correct or incorrect.

C.

Summary Introduction

To examine: Whether the statement “Clones isolated from cDNA libraries contain promoter sequences” is correct or incorrect.

D.

Summary Introduction

To explain: Whether the statement “PCR utilizes a heat stable DNA polymerase because for each amplification step, double-stranded DNA must be heat-denatured” is correct or incorrect.

E.

Summary Introduction

To examine: Whether the statement “Digestion of genomic DNA with Alu I, a restriction enzyme that recognizes a four-nucleotide sequence produces fragments that are all exactly 256 nucleotides in length” is correct or incorrect.

F.

Summary Introduction

To examine: Whether the statement “To make a cDNA library, both a DNA polymerase and a reverse transcriptase must be used” is correct or incorrect.

G.

Summary Introduction

To examine: Whether the statement “DNA fingerprinting by PCR relies on the fact that different individuals have different numbers of repeats in STR regions in their genome” is correct or incorrect.

H.

Summary Introduction

To examine: Whether the statement “It is possible for a coding region of a gene to be present in a genomic library prepared from a particular tissue but to be absent from a cDNA library prepared from the same” is correct or incorrect.

Expert Solution & Answer

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Answer 4

Question

Chapter 10, Problem 8Q

A.

Summary Introduction

To examine: Whether the statement “Restriction nucleases cut DNA at specific sites that are always located between genes” is correct or incorrect.

B.

Summary Introduction

To examine: Whether the statement “DNA migrates toward the positive electrode during electrophoresis” is correct or incorrect.

C.

Summary Introduction

To examine: Whether the statement “Clones isolated from cDNA libraries contain promoter sequences” is correct or incorrect.

D.

Summary Introduction

To explain: Whether the statement “PCR utilizes a heat stable DNA polymerase because for each amplification step, double-stranded DNA must be heat-denatured” is correct or incorrect.

E.

Summary Introduction

To examine: Whether the statement “Digestion of genomic DNA with Alu I, a restriction enzyme that recognizes a four-nucleotide sequence produces fragments that are all exactly 256 nucleotides in length” is correct or incorrect.

F.

Summary Introduction

To examine: Whether the statement “To make a cDNA library, both a DNA polymerase and a reverse transcriptase must be used” is correct or incorrect.

G.

Summary Introduction

To examine: Whether the statement “DNA fingerprinting by PCR relies on the fact that different individuals have different numbers of repeats in STR regions in their genome” is correct or incorrect.

H.

Summary Introduction

To examine: Whether the statement “It is possible for a coding region of a gene to be present in a genomic library prepared from a particular tissue but to be absent from a cDNA library prepared from the same” is correct or incorrect.

Expert Solution & Answer

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Answer 5

Chapter 10, Problem 8Q

A.

Summary Introduction

To examine: Whether the statement “Restriction nucleases cut DNA at specific sites that are always located between genes” is correct or incorrect.

B.

Summary Introduction

To examine: Whether the statement “DNA migrates toward the positive electrode during electrophoresis” is correct or incorrect.

C.

Summary Introduction

To examine: Whether the statement “Clones isolated from cDNA libraries contain promoter sequences” is correct or incorrect.

D.

Summary Introduction

To explain: Whether the statement “PCR utilizes a heat stable DNA polymerase because for each amplification step, double-stranded DNA must be heat-denatured” is correct or incorrect.

E.

Summary Introduction

To examine: Whether the statement “Digestion of genomic DNA with Alu I, a restriction enzyme that recognizes a four-nucleotide sequence produces fragments that are all exactly 256 nucleotides in length” is correct or incorrect.

F.

Summary Introduction

To examine: Whether the statement “To make a cDNA library, both a DNA polymerase and a reverse transcriptase must be used” is correct or incorrect.

G.

Summary Introduction

To examine: Whether the statement “DNA fingerprinting by PCR relies on the fact that different individuals have different numbers of repeats in STR regions in their genome” is correct or incorrect.

H.

Summary Introduction

To examine: Whether the statement “It is possible for a coding region of a gene to be present in a genomic library prepared from a particular tissue but to be absent from a cDNA library prepared from the same” is correct or incorrect.

Answer 6

Chapter 10, Problem 8Q

A.

Summary Introduction

To examine: Whether the statement “Restriction nucleases cut DNA at specific sites that are always located between genes” is correct or incorrect.

Answer 7

Chapter 10, Problem 8Q

A.

Summary Introduction

To examine: Whether the statement “Restriction nucleases cut DNA at specific sites that are always located between genes” is correct or incorrect.

Answer 8

B.

Summary Introduction

To examine: Whether the statement “DNA migrates toward the positive electrode during electrophoresis” is correct or incorrect.

Answer 9

B.

Summary Introduction

To examine: Whether the statement “DNA migrates toward the positive electrode during electrophoresis” is correct or incorrect.

Answer 10

C.

Summary Introduction

To examine: Whether the statement “Clones isolated from cDNA libraries contain promoter sequences” is correct or incorrect.

Answer 11

C.

Summary Introduction

To examine: Whether the statement “Clones isolated from cDNA libraries contain promoter sequences” is correct or incorrect.

Answer 12

D.

Summary Introduction

To explain: Whether the statement “PCR utilizes a heat stable DNA polymerase because for each amplification step, double-stranded DNA must be heat-denatured” is correct or incorrect.

Answer 13

D.

Summary Introduction

To explain: Whether the statement “PCR utilizes a heat stable DNA polymerase because for each amplification step, double-stranded DNA must be heat-denatured” is correct or incorrect.

Answer 14

E.

Summary Introduction

To examine: Whether the statement “Digestion of genomic DNA with Alu I, a restriction enzyme that recognizes a four-nucleotide sequence produces fragments that are all exactly 256 nucleotides in length” is correct or incorrect.

Answer 15

E.

Summary Introduction

To examine: Whether the statement “Digestion of genomic DNA with Alu I, a restriction enzyme that recognizes a four-nucleotide sequence produces fragments that are all exactly 256 nucleotides in length” is correct or incorrect.

Answer 16

F.

Summary Introduction

To examine: Whether the statement “To make a cDNA library, both a DNA polymerase and a reverse transcriptase must be used” is correct or incorrect.

Answer 17

F.

Summary Introduction

To examine: Whether the statement “To make a cDNA library, both a DNA polymerase and a reverse transcriptase must be used” is correct or incorrect.

Answer 18

G.

Summary Introduction

To examine: Whether the statement “DNA fingerprinting by PCR relies on the fact that different individuals have different numbers of repeats in STR regions in their genome” is correct or incorrect.

Answer 19

G.

Summary Introduction

To examine: Whether the statement “DNA fingerprinting by PCR relies on the fact that different individuals have different numbers of repeats in STR regions in their genome” is correct or incorrect.

Answer 20

H.

Summary Introduction

To examine: Whether the statement “It is possible for a coding region of a gene to be present in a genomic library prepared from a particular tissue but to be absent from a cDNA library prepared from the same” is correct or incorrect.

Answer 21

H.

Summary Introduction

To examine: Whether the statement “It is possible for a coding region of a gene to be present in a genomic library prepared from a particular tissue but to be absent from a cDNA library prepared from the same” is correct or incorrect.

Answer 22

Expert Solution & Answer

Answer 23

Expert Solution & Answer

Answer 24

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Answer 25

Ch. 10 - Prob. 1Q Ch. 10 - Prob. 2Q Ch. 10 - Prob. 3Q Ch. 10 - Prob. 4Q Ch. 10 - Prob. 5Q Ch. 10 - Prob. 6Q Ch. 10 - Prob. 7Q Ch. 10 - Prob. 8Q Ch. 10 - Prob. 9Q Ch. 10 - Prob. 10Q

To examine: Whether the statement “Restriction nucleases cut DNA at specific sites that are always located between genes” is correct or incorrect.

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To examine: Whether the statement “Restriction nucleases cut DNA at specific sites that are always located between genes” is correct or incorrect.

To examine: Whether the statement “DNA migrates toward the positive electrode during electrophoresis” is correct or incorrect.

To examine: Whether the statement “Clones isolated from cDNA libraries contain promoter sequences” is correct or incorrect.

To explain: Whether the statement “PCR utilizes a heat stable DNA polymerase because for each amplification step, double-stranded DNA must be heat-denatured” is correct or incorrect.

To examine: Whether the statement “Digestion of genomic DNA with Alu I, a restriction enzyme that recognizes a four-nucleotide sequence produces fragments that are all exactly 256 nucleotides in length” is correct or incorrect.

To examine: Whether the statement “To make a cDNA library, both a DNA polymerase and a reverse transcriptase must be used” is correct or incorrect.

To examine: Whether the statement “DNA fingerprinting by PCR relies on the fact that different individuals have different numbers of repeats in STR regions in their genome” is correct or incorrect.

To examine: Whether the statement “It is possible for a coding region of a gene to be present in a genomic library prepared from a particular tissue but to be absent from a cDNA library prepared from the same” is correct or incorrect.

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Chapter 10 Solutions