Which of the following is/are not essential to carry out the polymerase chain reaction? a. primers b. DNA polymerase c. gel electrophoresis d. high temperature

Question

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Answer 1

Textbook Question

Chapter 10, Problem 1MCQ

Which of the following is/are not essential to carry out the polymerase chain reaction?

a. primers
b. DNA polymerase
c. gel electrophoresis
d. high temperature

Expert Solution & Answer

Summary Introduction

Introduction:

PCR is a technique used to replicate and produce several copies of DNA. PCR has a wide application in research, medicine, agriculture, and many other fields.

Answer to Problem 1MCQ

Correct answer:

Gel electrophoresis is not essential to carry out polymerase chain reaction. Therefore, option (c) is correct.

Option (c) is given as “gel electrophoresis”.

Explanation of Solution

Justify reason for the correct statement:

PCR is a technique used to amplify DNA molecules. This technique involves three steps: denaturation, annealing, and extension. In denaturation, double stranded DNA is heated athigh temperature94 °C and converted into single stranded DNA. After conversion into single stranded, primers get annealed into single stranded DNA. After primer annealing, DNA polymerase enzyme bind to the 3′ end of primer and nucleotides which are complementary to template strand.

After polymerase chain reaction, the resulted fragments are run into gel electrophoresis. Gel electrophoresis is used to separate DNA fragments according to the molecular weight. Thus, gel electrophoresis is not essential to carry out PCR.

Hence, option (c) is correct.

Justify reasons for the incorrect statements:

Option (a) is given as “primers”.

Primers are essential to carry out the polymerase chain reaction. Hence, it is a wrong answer.

Option (b) is given as “DNA polymerase”.

DNA polymerase is important to synthesize DNA fragment. Hence, it is a wrong answer.

Option (d) is given as “high temperature”.

High temperature is required for denaturation of DNA. Hence, it is a wrong answer.

Hence, options (a),(b), and(d) are incorrect.

Conclusion

Gel electrophoresis technique is used after the PCR reaction. Gel electrophoresis technique is used to separate the amplified DNA fragments.

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Answer 2

Textbook Question

Chapter 10, Problem 1MCQ

Which of the following is/are not essential to carry out the polymerase chain reaction?

a. primers
b. DNA polymerase
c. gel electrophoresis
d. high temperature

Expert Solution & Answer

Summary Introduction

Introduction:

PCR is a technique used to replicate and produce several copies of DNA. PCR has a wide application in research, medicine, agriculture, and many other fields.

Answer to Problem 1MCQ

Correct answer:

Gel electrophoresis is not essential to carry out polymerase chain reaction. Therefore, option (c) is correct.

Option (c) is given as “gel electrophoresis”.

Explanation of Solution

Justify reason for the correct statement:

PCR is a technique used to amplify DNA molecules. This technique involves three steps: denaturation, annealing, and extension. In denaturation, double stranded DNA is heated athigh temperature94 °C and converted into single stranded DNA. After conversion into single stranded, primers get annealed into single stranded DNA. After primer annealing, DNA polymerase enzyme bind to the 3′ end of primer and nucleotides which are complementary to template strand.

After polymerase chain reaction, the resulted fragments are run into gel electrophoresis. Gel electrophoresis is used to separate DNA fragments according to the molecular weight. Thus, gel electrophoresis is not essential to carry out PCR.

Hence, option (c) is correct.

Justify reasons for the incorrect statements:

Option (a) is given as “primers”.

Primers are essential to carry out the polymerase chain reaction. Hence, it is a wrong answer.

Option (b) is given as “DNA polymerase”.

DNA polymerase is important to synthesize DNA fragment. Hence, it is a wrong answer.

Option (d) is given as “high temperature”.

High temperature is required for denaturation of DNA. Hence, it is a wrong answer.

Hence, options (a),(b), and(d) are incorrect.

Conclusion

Gel electrophoresis technique is used after the PCR reaction. Gel electrophoresis technique is used to separate the amplified DNA fragments.

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Answer 3

Textbook Question

Chapter 10, Problem 1MCQ

Which of the following is/are not essential to carry out the polymerase chain reaction?

a. primers
b. DNA polymerase
c. gel electrophoresis
d. high temperature

Expert Solution & Answer

Summary Introduction

Introduction:

PCR is a technique used to replicate and produce several copies of DNA. PCR has a wide application in research, medicine, agriculture, and many other fields.

Answer to Problem 1MCQ

Correct answer:

Gel electrophoresis is not essential to carry out polymerase chain reaction. Therefore, option (c) is correct.

Option (c) is given as “gel electrophoresis”.

Explanation of Solution

Justify reason for the correct statement:

PCR is a technique used to amplify DNA molecules. This technique involves three steps: denaturation, annealing, and extension. In denaturation, double stranded DNA is heated athigh temperature94 °C and converted into single stranded DNA. After conversion into single stranded, primers get annealed into single stranded DNA. After primer annealing, DNA polymerase enzyme bind to the 3′ end of primer and nucleotides which are complementary to template strand.

After polymerase chain reaction, the resulted fragments are run into gel electrophoresis. Gel electrophoresis is used to separate DNA fragments according to the molecular weight. Thus, gel electrophoresis is not essential to carry out PCR.

Hence, option (c) is correct.

Justify reasons for the incorrect statements:

Option (a) is given as “primers”.

Primers are essential to carry out the polymerase chain reaction. Hence, it is a wrong answer.

Option (b) is given as “DNA polymerase”.

DNA polymerase is important to synthesize DNA fragment. Hence, it is a wrong answer.

Option (d) is given as “high temperature”.

High temperature is required for denaturation of DNA. Hence, it is a wrong answer.

Hence, options (a),(b), and(d) are incorrect.

Conclusion

Gel electrophoresis technique is used after the PCR reaction. Gel electrophoresis technique is used to separate the amplified DNA fragments.

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Answer 4

Textbook Question

Chapter 10, Problem 1MCQ

Which of the following is/are not essential to carry out the polymerase chain reaction?

a. primers
b. DNA polymerase
c. gel electrophoresis
d. high temperature

Expert Solution & Answer

Summary Introduction

Introduction:

PCR is a technique used to replicate and produce several copies of DNA. PCR has a wide application in research, medicine, agriculture, and many other fields.

Answer to Problem 1MCQ

Correct answer:

Gel electrophoresis is not essential to carry out polymerase chain reaction. Therefore, option (c) is correct.

Option (c) is given as “gel electrophoresis”.

Explanation of Solution

Justify reason for the correct statement:

PCR is a technique used to amplify DNA molecules. This technique involves three steps: denaturation, annealing, and extension. In denaturation, double stranded DNA is heated athigh temperature94 °C and converted into single stranded DNA. After conversion into single stranded, primers get annealed into single stranded DNA. After primer annealing, DNA polymerase enzyme bind to the 3′ end of primer and nucleotides which are complementary to template strand.

After polymerase chain reaction, the resulted fragments are run into gel electrophoresis. Gel electrophoresis is used to separate DNA fragments according to the molecular weight. Thus, gel electrophoresis is not essential to carry out PCR.

Hence, option (c) is correct.

Justify reasons for the incorrect statements:

Option (a) is given as “primers”.

Primers are essential to carry out the polymerase chain reaction. Hence, it is a wrong answer.

Option (b) is given as “DNA polymerase”.

DNA polymerase is important to synthesize DNA fragment. Hence, it is a wrong answer.

Option (d) is given as “high temperature”.

High temperature is required for denaturation of DNA. Hence, it is a wrong answer.

Hence, options (a),(b), and(d) are incorrect.

Conclusion

Gel electrophoresis technique is used after the PCR reaction. Gel electrophoresis technique is used to separate the amplified DNA fragments.

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Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 5

Textbook Question

Answer 6

Textbook Question

Answer 7

Expert Solution & Answer

Summary Introduction

Introduction:

PCR is a technique used to replicate and produce several copies of DNA. PCR has a wide application in research, medicine, agriculture, and many other fields.

Answer to Problem 1MCQ

Correct answer:

Gel electrophoresis is not essential to carry out polymerase chain reaction. Therefore, option (c) is correct.

Option (c) is given as “gel electrophoresis”.

Explanation of Solution

Justify reason for the correct statement:

PCR is a technique used to amplify DNA molecules. This technique involves three steps: denaturation, annealing, and extension. In denaturation, double stranded DNA is heated athigh temperature94 °C and converted into single stranded DNA. After conversion into single stranded, primers get annealed into single stranded DNA. After primer annealing, DNA polymerase enzyme bind to the 3′ end of primer and nucleotides which are complementary to template strand.

After polymerase chain reaction, the resulted fragments are run into gel electrophoresis. Gel electrophoresis is used to separate DNA fragments according to the molecular weight. Thus, gel electrophoresis is not essential to carry out PCR.

Hence, option (c) is correct.

Justify reasons for the incorrect statements:

Option (a) is given as “primers”.

Primers are essential to carry out the polymerase chain reaction. Hence, it is a wrong answer.

Option (b) is given as “DNA polymerase”.

DNA polymerase is important to synthesize DNA fragment. Hence, it is a wrong answer.

Option (d) is given as “high temperature”.

High temperature is required for denaturation of DNA. Hence, it is a wrong answer.

Hence, options (a),(b), and(d) are incorrect.

Conclusion

Gel electrophoresis technique is used after the PCR reaction. Gel electrophoresis technique is used to separate the amplified DNA fragments.

Answer 8

Expert Solution & Answer

Summary Introduction

Introduction:

PCR is a technique used to replicate and produce several copies of DNA. PCR has a wide application in research, medicine, agriculture, and many other fields.

Answer to Problem 1MCQ

Correct answer:

Gel electrophoresis is not essential to carry out polymerase chain reaction. Therefore, option (c) is correct.

Option (c) is given as “gel electrophoresis”.

Explanation of Solution

Justify reason for the correct statement:

PCR is a technique used to amplify DNA molecules. This technique involves three steps: denaturation, annealing, and extension. In denaturation, double stranded DNA is heated athigh temperature94 °C and converted into single stranded DNA. After conversion into single stranded, primers get annealed into single stranded DNA. After primer annealing, DNA polymerase enzyme bind to the 3′ end of primer and nucleotides which are complementary to template strand.

After polymerase chain reaction, the resulted fragments are run into gel electrophoresis. Gel electrophoresis is used to separate DNA fragments according to the molecular weight. Thus, gel electrophoresis is not essential to carry out PCR.

Hence, option (c) is correct.

Justify reasons for the incorrect statements:

Option (a) is given as “primers”.

Primers are essential to carry out the polymerase chain reaction. Hence, it is a wrong answer.

Option (b) is given as “DNA polymerase”.

DNA polymerase is important to synthesize DNA fragment. Hence, it is a wrong answer.

Option (d) is given as “high temperature”.

High temperature is required for denaturation of DNA. Hence, it is a wrong answer.

Hence, options (a),(b), and(d) are incorrect.

Conclusion

Gel electrophoresis technique is used after the PCR reaction. Gel electrophoresis technique is used to separate the amplified DNA fragments.

Answer 9

Expert Solution & Answer

Answer 10

Expert Solution & Answer

Answer 11

Summary Introduction

Introduction:

PCR is a technique used to replicate and produce several copies of DNA. PCR has a wide application in research, medicine, agriculture, and many other fields.

Answer 12

Answer to Problem 1MCQ

Correct answer:

Gel electrophoresis is not essential to carry out polymerase chain reaction. Therefore, option (c) is correct.

Option (c) is given as “gel electrophoresis”.

Answer 13

Explanation of Solution

Justify reason for the correct statement:

PCR is a technique used to amplify DNA molecules. This technique involves three steps: denaturation, annealing, and extension. In denaturation, double stranded DNA is heated athigh temperature94 °C and converted into single stranded DNA. After conversion into single stranded, primers get annealed into single stranded DNA. After primer annealing, DNA polymerase enzyme bind to the 3′ end of primer and nucleotides which are complementary to template strand.

After polymerase chain reaction, the resulted fragments are run into gel electrophoresis. Gel electrophoresis is used to separate DNA fragments according to the molecular weight. Thus, gel electrophoresis is not essential to carry out PCR.

Hence, option (c) is correct.

Justify reasons for the incorrect statements:

Option (a) is given as “primers”.

Primers are essential to carry out the polymerase chain reaction. Hence, it is a wrong answer.

Option (b) is given as “DNA polymerase”.

DNA polymerase is important to synthesize DNA fragment. Hence, it is a wrong answer.

Option (d) is given as “high temperature”.

High temperature is required for denaturation of DNA. Hence, it is a wrong answer.

Hence, options (a),(b), and(d) are incorrect.

Answer 14

Conclusion

Gel electrophoresis technique is used after the PCR reaction. Gel electrophoresis technique is used to separate the amplified DNA fragments.

Answer 15

Ch. 10.1 - Provide examples of practical applications of...Ch. 10.2 - Prob. 2AYP Ch. 10.2 - Describe how gel electrophoresis is used to...Ch. 10.2 - Prob. 4AYP Ch. 10.2 - Prob. 5AYP Ch. 10.3 - Prob. 6AYP Ch. 10.3 - List examples of genetically modified bacteria,...Ch. 10.4 - Prob. 8AYP Ch. 10.4 - Prob. 9AYP Ch. 10.5 - Outline in general terms the process of DNA...

Which of the following is/are not essential to carry out the polymerase chain reaction? a. primers b. DNA polymerase c. gel electrophoresis d. high temperature

Solution Summary: The author explains that PCR is a technique used to replicate and produce several copies of DNA. Gel electrophoresis is not essential to carry out polymerase chain reaction.

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Answer to Problem 1MCQ

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