Biochemistry
8th Edition
ISBN: 9781285429106
Author: Campbell, Mary K., FARRELL, Shawn O.
Publisher: Cengage Learning,
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Chapter 10, Problem 18RE
REFLECT AND APPLY Why is it not surprising that the addition of
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Chapter 10 Solutions
Biochemistry
Ch. 10 - RECALL Define replication, transcription, and...Ch. 10 - REFLECT AND APPLY Is the following statement true...Ch. 10 - REFLECT AND APPLY Why is it more important for DNA...Ch. 10 - RECALL Why is the replication of DNA referred to...Ch. 10 - RECALL What is a replication fork? Why is it...Ch. 10 - Prob. 6RECh. 10 - RECALL Why is it necessary to unwind the DNA helix...Ch. 10 - REFLECT AND APPLY In the MeselsonStahl experiment...Ch. 10 - REFLECT AND APPLY Suggest a reason why it would be...Ch. 10 - RECALL Do DNA-polymerase enzymes also function as...
Ch. 10 - RECALL Compare and contrast the properties of the...Ch. 10 - REFLECT AND APPLY Define processivity, and...Ch. 10 - REFLECT AND APPLY Comment on the dual role of the...Ch. 10 - REFLECT AND APPLY What is the importance of...Ch. 10 - REFLECT AND APPLY DNA synthesis always takes place...Ch. 10 - REFLECT AND APPLY What would happen to the...Ch. 10 - Prob. 17RECh. 10 - REFLECT AND APPLY Why is it not surprising that...Ch. 10 - Prob. 19RECh. 10 - RECALL List the substances required for...Ch. 10 - RECALL Describe the discontinuous synthesis of the...Ch. 10 - RECALL What are the functions of the gyrase,...Ch. 10 - RECALL Single-stranded regions of DNA are attacked...Ch. 10 - RECALL Describe the role of DNA ligase in the...Ch. 10 - RECALL What is the primer in DNA replication?Ch. 10 - Prob. 26RECh. 10 - REFLECT AND APPLY Why is a short RNA primer needed...Ch. 10 - Prob. 28RECh. 10 - RECALL What was the recent change in the estimated...Ch. 10 - Prob. 30RECh. 10 - RECALL Why is a clamp loader necessary in...Ch. 10 - RECALL How does proofreading take place in the...Ch. 10 - Prob. 33RECh. 10 - Prob. 34RECh. 10 - BIOCHEMICAL CONNECTIONS Of what benefit is it for...Ch. 10 - REFLECT AND APPLY Your book contains about 2...Ch. 10 - REFLECT AND APPLY E. coli incorporates...Ch. 10 - REFLECT AND APPLY Given the typing speed from...Ch. 10 - Prob. 39RECh. 10 - REFLECT AND APPLY How can breakdown in DNA repair...Ch. 10 - Prob. 41RECh. 10 - RECALL What is a direct way of repairing...Ch. 10 - Prob. 43RECh. 10 - Prob. 44RECh. 10 - Prob. 45RECh. 10 - Prob. 46RECh. 10 - RECALL How did Messelson and Weigle demonstrate...Ch. 10 - Prob. 48RECh. 10 - RECALL What is the Holliday Model?Ch. 10 - RECALL Do eukaryotes have fewer origins of...Ch. 10 - RECALL How does DNA replication in eukaryotes...Ch. 10 - Prob. 52RECh. 10 - REFLECT AND APPLY (a) Eukaryotic DNA replication...Ch. 10 - Prob. 54RECh. 10 - Prob. 55RECh. 10 - Prob. 56RECh. 10 - Prob. 57RECh. 10 - Prob. 58RECh. 10 - Prob. 59RECh. 10 - Prob. 60RECh. 10 - Prob. 61RECh. 10 - Prob. 62RECh. 10 - Prob. 63RECh. 10 - Prob. 64RE
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- 1. Write the transamination reaction for alanine. Indicate what happens next to each of the molecules in the reaction, and under what conditions it happens. 2.arrow_forwardCH3 17. Which one of the compounds below is the HNO3 H2 1. NaNO2, HCI Br₂ 1. LiAlH4 major organic product H2SO4 Ni 2. CuCN, KCN FeBr3 2. H₂O, H+ obtained from the following series of reactions? CH3 toluene CH3 CH3 Br Br Br CH3 CH3 && Br Br NH₂ A NH₂ NH₂ B C NH₂ ΝΗΣ D Earrow_forward13. Which one of the compounds below is the major organic product obtained from the following series of reactions? A + H2C=CH-CO Me heat (CH3)2NH 1. LiAlH4 2. H₂O ? 1,3-butadiene OH 'N' B C 14. Which one of the compounds below is the major organic product obtained from the following series of reactions? 'N' D 'N' E 1. XS CH3I 'N' 2. Ag₂O 3. H₂O, A A B с D E Narrow_forward
- A NH2 NH2 B C H₂N. NH₂ D 5. The five compounds above all have molecular weights close to 75 g/mol. Which one has the highest boiling point? 6. The five compounds above all have molecular weights close to 75 g/mol. Which one has the lowest boiling point? E NH₂arrow_forward7. A solution of aniline in diethyl ether is added to a separatory funnel. Which ONE of the following aqueous solutions will remove aniline from the ether layer (and transfer it to the aqueous layer) if it is added to the separatory funnel and the funnel is shaken? A) aqueous NH3 B) aqueous Na2CO3 C) aqueous HCl D) aqueous NaOH E) aqueous CH3COONa (sodium acetate)arrow_forward11. Which of the following choices is the best reagent to use to perform the following conversion? A) 1. LiAlH4 2. H₂O B) HCI, H₂O D) NaOH, H₂O E) Na№3 ? 'N' 'N' C) 1. CH3MgBr 2. HCI, H₂Oarrow_forward
- NH2 ΝΗΣ NH₂ NH2 NH2 A OCH3 NO₂ B C D E 1. Which one of the five compounds above is the strongest base? 2. Which one of the five compounds above is the second-strongest base? (That is, which one is next-to-strongest base?) 3. Which one of the five compounds above is the weakest base? 4. Which one of the five compounds above is the second-weakest base? (That is, which one is next-to-weakest base?) NO2arrow_forwardDraw a diagram to demonstrate 3' to 5' exonuclease activity?arrow_forward1. Write the piece of mRNA that would code for the amino acid sequence NH3 - met - tyr - cys- his- CO2. Label the 5' and 3' ends. (diagram attached) 2.arrow_forward
- You were tasked with purifying an untagged transcription factor (molecular weight 65,000Da, isoelectric point unknown) from a contaminant Protein A (molecular weight 50,000 Da, isoelectric point 5.0). You were also instructed to use the protein for crystallization studies after purification.i) In the initial purification step, which type of chromatography should you attempt? Explain your choice and specify the requirements of the buffer solution you should use. ii) Analyzing the proteins recovered from step i) using SDS-PAGE, you still observed a faint band of Protein A in addition to the transcription factor band. Given the limited time available for further purification, you must choose ONE appropriate chromatography method to maximize the chances of separating the two proteins as well as your crystallization studies. Provide a detailed explanation of your selection and the techniques/strategies you would employ to achieve this.arrow_forwardYou were given a mixture of two proteins with different isoelectric points and molecular weights:• Protein X: pI 4.2, MW 42,000• Protein Y: pI 9.8, MW 90,000Using a Tris-glycine discontinuous native gel (pH8.3) electrophoresis system with a running gel of 12%, only a single band was observed upon protein staining after electrophoresis. Explain the observed result and discuss possible factors affecting protein migration in this system.arrow_forwardThe standard cost of Product B manufactured by Oriole Company includes 3.5 units of direct materials at $5.40 per unit. During June, 27,300 units of direct materials are purchased at a cost of $5.15 per unit, and 27,300 units of direct materials are used to produce 7,600 units of Product B. (a) Compute the total materials variance and the price and quantity variances. Total materials variance Materials price variance Materials quantity variance $ (b) Compute the total materials variance and the price and quantity variances, assuming the purchase price is $6.35 and the quantity purchased and used is 26,300 units. Total materials variance Materials price variance Materials quantity variance $arrow_forward
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