Biochemistry 410/411 Textbook - 5th Edition - Custom Texas A&M University
Biochemistry 410/411 Textbook - 5th Edition - Custom Texas A&M University
5th Edition
ISBN: 9781119302940
Author: Voet
Publisher: WILEY C
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Chapter 10, Problem 18CQ
Summary Introduction

To calculate: The time taken by 100 molecules of Valinomycin to transport enough K+ ions to change the concentration inside an erythrocyte of the given volume.

Concept introduction: Valinomycin is the characterized ionophore that specially binds to the K+ ion. It is a cyclic ionophore that contains ester linkage as well as peptide bonds, and D-amino acids and L-amino acids. Valinomycin is the channel-forming ionophore that forms transmembrane channels or pores through which the selected ions can pass through.

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The rate data from an enzyme catalyzed reaction with and without an inhibitor present is found in the image.  Question: what is the KM  and Vm and the nature of inhibition
1. Estimate the concentration of an enzyme within a living cell. Assume that:  (a): fresh tissue is 80% water and all of it is intracellular (b): the total soluble protein represents 15% of the weight (c): all the soluble proteins are enzymes (d): the average molecular weight of the proteins is 150,000 (E): about 100 different enzymes are present   please help I am lost
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