GENETIC ANALYSIS: INTEGRATED - ACCESS
3rd Edition
ISBN: 9780135349298
Author: Sanders
Publisher: PEARSON
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Chapter 1, Problem 25P
Summary Introduction
To review:
The size of a DNA fragment affects its electrophoretic mobility on the electrophoresis gels.
Introduction:
Electrophoretic mobility is the migration of charged particles under the influence of applied electrical field. The cations move toward the negatively charged cathode, anions move toward the positively charged anode, and neutral species remain stationary.
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You’re curious about the effect that gap genes have on the pair-rule gene, evenskipped (eve), so you isolate and sequence each of the eve enhancers. You’re particularly interested in one of the enhancers, which is just upstream of the eve gene.
Describe an experimental technique you would use to find out where this particular eve enhancer is active.
For short answer
questions, write your
answers on the line
provided. To the right is
the mRNA codon table
to use as needed
throughout the exam.
First letter
U
บบบ
U CA
UUCPhe
UUA
UCU
Phe UCC
UUG Leu
CUU
UAU.
G
U
UAC TV UGCys
UAA Stop UGA Stop A
UAG Stop UGG Trp
Ser
UCA
UCG
CCU]
0
CUC
CUA
CCC
CAC
CAU His
CGU
CGC
Leu
Pro
CCA
CAA
Gin
CGA Arg
CUG
CCG
CAG
CGG
AUU
ACU
AAU T
AUC lle
A
1
ACC
Thr
AUA
ACA
AUG
Mot ACG
AGG Arg
GUU
GCU
GUC
GCC
G
Val
Ala
GAC Asp
GGU
GGC
GUA
GUG
GCA
GCG
GAA
GGA
Gly
Glu
GAGJ
GGG
AACASH AGU Ser
AAA1
AAG Lys
GAU
AGA
CAL CALUCAO CAO
G
Third letter
1. (+7) Use the table below to answer the questions; use the codon table above to assist you.
The promoter sequence of DNA is on the LEFT. You do not need to fill in the entire table.
Assume we are in the middle of a gene sequence (no need to find a start codon).
DNA
1
DNA
2
mRNA
tRNA
Polypeptide
C
Val
G
C.
T
A
C
a. On which strand of DNA is the template strand (DNA 1 or 2)?_
b. On which side of the mRNA is the 5' end (left or…
3. (6 pts) Fill in the boxes according to the directions on the right.
Structure
R-C
R-COOH
OH
R-OH
i
R-CO-R' R
R-PO4
R-CH3
C.
0
R'
R-O-P-OH
1
OH
H
R-C-H
R-N'
I-
H
H
R-NH₂
\H
Name
Properties
Chapter 1 Solutions
GENETIC ANALYSIS: INTEGRATED - ACCESS
Ch. 1 - 1. Genetics affects many aspects of our lives....Ch. 1 - 2. How do you think the determination that DNA is...Ch. 1 - 3. A commentator once described genetics as “the...Ch. 1 - All life shares DNA as the hereditary material....Ch. 1 - Define the terms allele, chromosome, and gene and...Ch. 1 - 6. Define the terms genotype and phenotype, and...Ch. 1 - 7. Define natural selection, and describe how...Ch. 1 - Describe the modern synthesis of evolution, and...Ch. 1 - What are the four processes of evolution? Briefly...Ch. 1 - Define each of the following terms: a....
Ch. 1 - 11. Compare and contrast the genome, the proteome,...Ch. 1 - With respect to transcription describe the...Ch. 1 - Plant agriculture and animal domestication...Ch. 1 - Briefly describe the contribution each of the...Ch. 1 - If thymine makes up 21% of the DNA nucleotides in...Ch. 1 - What reactive chemical groups are found at the 5...Ch. 1 - Identify two differences in chemical composition...Ch. 1 - What is the central dogma of molecular biology?...Ch. 1 - A portion of a polypeptide contains the amino...Ch. 1 - The following segment of DNA is the template...Ch. 1 - 23. Fill in the missing nucleotides (so there are...Ch. 1 - 24. Suppose a genotype for a protein-producing...Ch. 1 - Prob. 25PCh. 1 - 26. Four nucleic acid samples are analyzed to...Ch. 1 - 27. What is meant by the term homology? How is...Ch. 1 - 28. If one is constructing a phylogeny of reptiles...Ch. 1 - 29. Consider the following segment of...Ch. 1 - 30. Ethical and social issues have become a large...Ch. 1 - 31. In certain cases, genetic testing can identify...Ch. 1 - 32. What information presented in this chapter and...Ch. 1 - 33. It is common to study the biology and genetics...
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