Human Anatomy
6th Edition
ISBN: 9781260399820
Author: SALADIN, Kenneth
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Chapter 1, Problem 1.1.4AYLO
To test your knowledge, discuss the following topics with a study partner or in writing ideally from. memory.
4. Ways in which dissection differs from exploratory surgery, and why exploratory surgery is far less common now than it was in the 1950s.
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 1 Solutions
Human Anatomy
Ch. 1.1 - The concept of MRI was conceived in 1948 but could...Ch. 1.1 - Prob. 2AWYKCh. 1.1 - Answer the following questions to test your...Ch. 1.1 - Anwer the following questions to test your...Ch. 1.1 - Anwer the following questions to test your...Ch. 1.1 - Anwer the following questions to test your...Ch. 1.2 - Anwer the following questions to test your...Ch. 1.2 - Anwer the following questions to test your...Ch. 1.2 - Prob. 7BYGOCh. 1.2 - Prob. 8BYGO
Ch. 1.2 - Prob. 9BYGOCh. 1.3 - Anwer the following questions to test your...Ch. 1.3 - Anwer the following questions to test your...Ch. 1.3 - Prob. 12BYGOCh. 1.3 - Prob. 13BYGOCh. 1 - To test your knowledge, discuss the following...Ch. 1 - Prob. 1.1.2AYLOCh. 1 - To test your knowledge, discuss the following...Ch. 1 - To test your knowledge, discuss the following...Ch. 1 - To test your knowledge, discuss the following...Ch. 1 - To test your knowledge, discuss the following...Ch. 1 - To test your knowledge, discuss the following...Ch. 1 - To test your knowledge, discuss the following...Ch. 1 - Prob. 1.2.2AYLOCh. 1 - Prob. 1.2.3AYLOCh. 1 - Prob. 1.2.4AYLOCh. 1 - To test your knowledge, discuss the following...Ch. 1 - Prob. 1.2.6AYLOCh. 1 - To test your knowledge, discuss the following...Ch. 1 - To test your knowledge, discuss the following...Ch. 1 - Prob. 1.2.9AYLOCh. 1 - To test your knowledge, discuss the following...Ch. 1 - Prob. 1.2.11AYLOCh. 1 - To test your knowledge, discuss the following...Ch. 1 - Prob. 1.2.13AYLOCh. 1 - To test your knowledge, discuss the following...Ch. 1 - To test your knowledge, discuss the following...Ch. 1 - Prob. 1.2.16AYLOCh. 1 - Prob. 1.2.17AYLOCh. 1 - To test your knowledge, discuss the following...Ch. 1 - Prob. 1.3.2AYLOCh. 1 - To test your knowledge, discuss the following...Ch. 1 - Prob. 1.3.4AYLOCh. 1 - Prob. 1.3.5AYLOCh. 1 - Prob. 1.3.6AYLOCh. 1 - Prob. 1.3.7AYLOCh. 1 - Structure that can be observed with the naked eye...Ch. 1 - Prob. 2TYRCh. 1 - Prob. 3TYRCh. 1 - Prob. 4TYRCh. 1 - Prob. 5TYRCh. 1 - Which of these regions is not part of the upper...Ch. 1 - Prob. 7TYRCh. 1 - Which of these is not an organ sytem? muscular...Ch. 1 - Prob. 9TYRCh. 1 - An imaging technique that exposes the patient to...Ch. 1 - Prob. 11TYRCh. 1 - The forearm is said to be ________ when the palms...Ch. 1 - Prob. 13TYRCh. 1 - Prob. 14TYRCh. 1 - Prob. 15TYRCh. 1 - Prob. 16TYRCh. 1 - Prob. 17TYRCh. 1 - Prob. 18TYRCh. 1 - Prob. 19TYRCh. 1 - Prob. 20TYRCh. 1 - Prob. 1BYMVCh. 1 - State a meaning of each word element and give a...Ch. 1 - Prob. 3BYMVCh. 1 - Prob. 4BYMVCh. 1 - Prob. 5BYMVCh. 1 - Prob. 6BYMVCh. 1 - Prob. 7BYMVCh. 1 - Prob. 8BYMVCh. 1 - Prob. 9BYMVCh. 1 - Prob. 10BYMVCh. 1 - Prob. 1WWWTSCh. 1 - Prob. 2WWWTSCh. 1 - Briefly explain why each of the following...Ch. 1 - Prob. 4WWWTSCh. 1 - Prob. 5WWWTSCh. 1 - Prob. 6WWWTSCh. 1 - Briefly explain why each of the following...Ch. 1 - Prob. 8WWWTSCh. 1 - Prob. 9WWWTSCh. 1 - Prob. 10WWWTSCh. 1 - Classify each of the following radiologic...Ch. 1 - Prob. 2TYCCh. 1 - Prob. 3TYCCh. 1 - Prob. 4TYCCh. 1 - Why do you think the writers of Terminologia...
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