You seed a 10cm tissue culture plate with a 4x10^6 HeLa cells. HeLa fells have a doubling time of 24h. At 9am on Tuesday you wish to infect the cells with poliovirus at an MOI of 10. Your poliovirus stock is at a concentration of 4x10^7 pfu/ml. What volume of your poliovirus stock do you need to add to your plate of cells?
You seed a 10cm tissue culture plate with a 4x10^6 HeLa cells. HeLa fells have a doubling time of 24h. At 9am on Tuesday you wish to infect the cells with poliovirus at an MOI of 10. Your poliovirus stock is at a concentration of 4x10^7 pfu/ml. What volume of your poliovirus stock do you need to add to your plate of cells?
Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Chapter17: Bacterial And Viral Genetics
Section: Chapter Questions
Problem 7TYK: When a virus enters the lysogenic stage: a. the viral DNA is replicated outside the host cell. b. it...
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4. You seed a 10cm tissue culture plate with a 4x10^6 HeLa cells. HeLa fells have a doubling time of 24h. At 9am on Tuesday you wish to infect the cells with poliovirus at an MOI of 10. Your poliovirus stock is at a concentration of 4x10^7 pfu/ml. What volume of your poliovirus stock do you need to add to your plate of cells?
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