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You perform a series of experiments on the synthesis of the pituitary hormone prolactin, which is a single polypeptide chain 199 amino acids long. In the first experiment, the mRNA coding for prolactin is translated in a cell-free protein synthesizing system containing ribosomes, amino acids, RNAS, aminoacyl-tRNA synthetases, ATP, GTP, and the appropriate initiation, elongation, and termination factors. Under these conditions, a polypeptide chain 227 amino acids long is synthesized. In a second experiment, the mRNA is translated in the same cell-free system to which you have added SRP. In this case, translation stops after a peptide about 70 amino acids long has been produced. In a third experiment, you use the same cell-free translation system to which you have added both SRP and endoplasmic reticulum vesicles, and you find that that the prolactin MRNA now produces a polypeptide that is 199 amino acids long. A) Explain these results. B) Where in the cell-free system would you expect to find the polypeptide produced in experiment 3?

You perform a series of experiments on the synthesis of the pituitary hormone prolactin, which is a single polypeptide chain 199 amino acids long. In the first experiment, the mRNA coding for prolactin is translated in a cell-free protein synthesizing system containing ribosomes, amino acids, RNAS, aminoacyl-tRNA synthetases, ATP, GTP, and the appropriate initiation, elongation, and termination factors. Under these conditions, a polypeptide chain 227 amino acids long is synthesized. In a second experiment, the mRNA is translated in the same cell-free system to which you have added SRP. In this case, translation stops after a peptide about 70 amino acids long has been produced. In a third experiment, you use the same cell-free translation system to which you have added both SRP and endoplasmic reticulum vesicles, and you find that that the prolactin MRNA now produces a polypeptide that is 199 amino acids long. A) Explain these results. B) Where in the cell-free system would you expect to find the polypeptide produced in experiment 3?

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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2. You perform a series of experiments on the synthesis of the pituitary hormone prolactin, which is a single polypeptide chain 199 amino acids long. In the first experiment, the mRNA coding for prolactin is translated in a cell-free protein synthesizing system containing ribosomes, amino acids, TRNAS, aminoacyl-tRNA synthetases, ATP, GTP, and the appropriate initiation, elongation, and termination factors. Under these conditions, a polypeptide chain 227 amino acids long is synthesized. In a second experiment, the mRNA is translated in the same cell-free system to which you have added SRP. In this case, translation stops after a peptide about 70 amino acids long has been produced. In a third experiment, you use the same cell-free translation system to which you have added both SRP and endoplasmic reticulum vesicles, and you find that that the prolactin MRNA now produces a polypeptide that is 199 amino acids long. A) Explain these results. B) Where in the cell-free system would you expect to find the polypeptide produced in experiment 3?
Transcribed Image Text:2. You perform a series of experiments on the synthesis of the pituitary hormone prolactin, which is a single polypeptide chain 199 amino acids long. In the first experiment, the mRNA coding for prolactin is translated in a cell-free protein synthesizing system containing ribosomes, amino acids, TRNAS, aminoacyl-tRNA synthetases, ATP, GTP, and the appropriate initiation, elongation, and termination factors. Under these conditions, a polypeptide chain 227 amino acids long is synthesized. In a second experiment, the mRNA is translated in the same cell-free system to which you have added SRP. In this case, translation stops after a peptide about 70 amino acids long has been produced. In a third experiment, you use the same cell-free translation system to which you have added both SRP and endoplasmic reticulum vesicles, and you find that that the prolactin MRNA now produces a polypeptide that is 199 amino acids long. A) Explain these results. B) Where in the cell-free system would you expect to find the polypeptide produced in experiment 3?
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