13 Below is a diagram of a gene that is not normally alternatively spliced. All four exons (represented as boxes) are found in the mature mRNA. Transcription starts at the arrowlabeled TSS, transcription stops at the line labeled TT, and the promoter location is indicated by the line. The indicated DNA sequences for two individuals are found withinexon 2 as indicated, with Individual 2 having a mutation. Both sequences are in-frame with the upstream start codon.PromoterTSSATG(Start codon)TAA(Stop codon)Individual 1 5' ACAGGAGGAATAAGTTATGCAIndividual 2 5' ACAGGAGGAATAAGTTAAGCA3'3'TT You isolate the mature mRNAs for each individual and run them on a northern blot. Compared with the mRNA for Individual 1, you find that the mRNA for Individual 2 ismore abundant.the same length.shorter.O O O O Oalternatively spliced.longer.

Name: 13 Below is a diagram of a gene that is not normally alternatively spl
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Description: 13 Below is a diagram of a gene that is not normally alternatively spliced. All four exons (represented as boxes) are found in the mature mRNA. Transcription starts at the arrowlabeled TSS, transcription stops at the line labeled TT, and the promoter

It is a technique which is used to detect the gene expression by analysing the size and abundance or…

Answered: You isolate the mature mRNAs for each…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

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Below is a diagram of a gene that is not normally alternatively spliced. All four exons (represented as boxes) are found in the mature mRNA. Transcription starts at the arrow
labeled TSS, transcription stops at the line labeled TT, and the promoter location is indicated by the line. The indicated DNA sequences for two individuals are found within
exon 2 as indicated, with Individual 2 having a mutation. Both sequences are in-frame with the upstream start codon.
Promoter
TSS
ATG
(Start codon)
TAA
(Stop codon)
Individual 1 5' ACAGGAGGAATAAGTTATGCA
Individual 2 5' ACAGGAGGAATAAGTTAAGCA
3'
3'
TT

You isolate the mature mRNAs for each individual and run them on a northern blot. Compared with the mRNA for Individual 1, you find that the mRNA for Individual 2 is
more abundant.
the same length.
shorter.
O O O O O
alternatively spliced.
longer.

Expert Solution

Step 1: NORTHERN BLOTTING

It is a technique which is used to detect the gene expression by analysing the size and abundance or number of RNA molecules produced by the gene.
It provides information about gene expression
This is done by using some readymade RNA probes which are complementary to the mRNA produced by the gene
Much better techniques are available today for analysing gene expression today, like, microarray and RNA sequencing.
Northern blotting is still used today for estimating the size of RNA produced by a particular gene.

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