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You isolate a plasmid from E. coli that's 2100bp in length. What is the most likely superhelical state of this molecule? a. Positive Supercoils o b. Negative Supercoils O c. No Supercoiling

You isolate a plasmid from E. coli that's 2100bp in length. What is the most likely superhelical state of this molecule? a. Positive Supercoils o b. Negative Supercoils O c. No Supercoiling

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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You isolate a plasmid from E. coli that's 2100bp in length. What is the most likely superhelical state of this molecule? a. Positive Supercoils o b. Negative Supercoils c. No Supercoiling (Continued from Question 8) After treatment with topoisomerase I (assume cutting and re-joining work perfectly) what is the linking number, twist, and writhe of this molecule? Edit: assume that topoisomerase completely relaxes the molecule so that DNA follows its natural twist and there is no writhe. Linking Number Twist Writhe Continued from Question 9 You cut the molecule, overwind (add twist) the plasmid by 30 turns, rejoin and allow supercoils to form. What is the new linking number, twist, and writhe? Linking Number Twist Writhe
Transcribed Image Text:You isolate a plasmid from E. coli that's 2100bp in length. What is the most likely superhelical state of this molecule? a. Positive Supercoils o b. Negative Supercoils c. No Supercoiling (Continued from Question 8) After treatment with topoisomerase I (assume cutting and re-joining work perfectly) what is the linking number, twist, and writhe of this molecule? Edit: assume that topoisomerase completely relaxes the molecule so that DNA follows its natural twist and there is no writhe. Linking Number Twist Writhe Continued from Question 9 You cut the molecule, overwind (add twist) the plasmid by 30 turns, rejoin and allow supercoils to form. What is the new linking number, twist, and writhe? Linking Number Twist Writhe
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