Tris succinate buffermolecule and two carboxylate groups from the succinic acid/succinate.The buffer thus has three distinct pka's.Requirements:HOThe molar mass of Tris (CH₁NO) is 121.14 g/mol, that of succinate (C.H.O.) is 118.09 g/mol and themolar mass of Trisą succinate (2C4H11NO3 C₁H6O4 = C12H28N2010) is 2x121.14 g/mol + 118.09 g/mol =360.37 g/mol.Four 50 mL Falcon tubesWeighing paperCHYou have to calculate the Tris and succinate you have to weigh off to preparein 25ml. Tris = 0,7M and succinate = 0,35M. Calculate the concentration. Forthe 50ml?

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You have to calculate the Tris and succinate you have to weigh off to prepare in 25ml. Tris = 0,7M and succinate = 0,35M. Calculate the concentration. For the 50ml?

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

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Question

Tris succinate buffer
molecule and two carboxylate groups from the succinic acid/succinate.
The buffer thus has three distinct pka's.
Requirements:
HO
The molar mass of Tris (CH₁NO) is 121.14 g/mol, that of succinate (C.H.O.) is 118.09 g/mol and the
molar mass of Trisą succinate (2C4H11NO3 C₁H6O4 = C12H28N2010) is 2x121.14 g/mol + 118.09 g/mol =
360.37 g/mol.
Four 50 mL Falcon tubes
Weighing paper
CH
You have to calculate the Tris and succinate you have to weigh off to prepare
in 25ml. Tris = 0,7M and succinate = 0,35M. Calculate the concentration. For
the 50ml?

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