Answered: You have isolated a…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Draw and upload profile for the cells sorted at the restrictive temperature label the axes

Transcribed Image Text:You have isolated a temperature-sensitive cell-cycle mutant. The mutated gene encodes the activating subunit of the APC. The mutant protein is defective at high temperatures. You sort several thousand cells and recover the WT-looking profile below: *Graph Explanation:* The graph is a plot of cell counts (in thousands) against propidium iodide fluorescence. The x-axis represents propidium iodide fluorescence, ranging from 50 to 250. The y-axis represents the number of cells, with values ranging from 0 to 5 (in thousands). The graph shows two peaks: a large peak at approximately 100 fluorescence units, and a smaller one near 200 fluorescence units. Next, you grow the mutant at the restrictive high temperature and sort an equal number of cells. First, what phase of the cell cycle do you expect the cells to arrest in? - ○ G1 - ○ M - ○ G2 - ○ S

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