You have discovered a gene in mice that is similar to a gene in yeast. How might you determine whether this gene is essential for development in mice
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You have discovered a gene in mice that is similar to a gene in yeast. How might you determine whether this gene is essential for development in mice
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- How have we discovered that specific genes control development in an organism like Drosophila?Another way to study the role of proteins (e.g., transcription factors) that function in development is to microinject the mRNA that encodes a protein, or the purified protein itself, into an oocyte or embryo, and then determine how this affects the subsequent development of the embryo, larva, and adult. For example, if Bicoid protein is injected into the posterior region of an oocyte, the resulting embryo will develop into a larva that has anterior structures at both ends. Based on your understanding of the function of each developmental gene, what would be the predicted phenotype if the following proteins or mRNAs were injected into normal oocytes? A. Nanos mRNA injected into the anterior end of an oocyte B. Antp protein injected into the posterior end of an embryo C. Toll mRNA injected into the dorsal side of an early embryoYou inject bicoid MRNA into the posterior end of a fertilized fruit fly egg just prior to the first cleavage. How will the experiment affect Hox gene expression in this fly? How will it affect the fly embryo's anatomy? Explain your answer, demonstrating your understanding of the role bicoid and Hox genes play in development.
- given a situation in which you manipulated a zebrafish embryo by injecting high levels of retinoic acid so that no anterior genes will be expressed. Surprisingly, after embryonic development, you are still able to observed the presence of anterior structures. What could explain this observation? What changes in your experiment would you make to achieve your objective?You found a strain of mutant fruit flies (Drosophila) living on the rotten bananas in your dorm room. You notice that many of the larvae have abnormal abdominal segments. You want to know if the “abdomenless” mutation is a maternal effect gene. Describe an experiment you would do to determine this, and the results that would support and contradict the notion that the abdomenless gene encodes a maternal determinant.Scientists have cloned some animals by injecting a nucleus from an early embryo into an enucleated egg cell. Does this outcome demonstrate that genetic material is not lost during development? Why or why not?
- You have identified a Drosophila gene that is expressed exclusively in the odd-numbered "stripes" in the cellular blastoderm. Assuming that this gene is not redundant, what would be the most likely phenotype cause by a loss-of-function mutation in this gene? an embryo missing odd numbered segments an adult fly with a second pair of wings instead of halteres an embryo with two anterior ends an embryo missing even-numbered segments an embryo missing larval segments 3-10Your friend can't understand how it is possible to combine a somatic cell from their pet with an oocyte from a different animal and create a clone of their pet. Won't the animal that develops be the offspring of the two animals rather than a clone of the animal that donated the somatic cell? Explain to your friend why this is not the case.You are studying Hox genes in crane flies (Leptotarsus testaceus). The cranefly genome is sequenced, and in craneflies. Using your understanding of Hox genes, design an experiment testing where the homolog of the EVE gene is expressed in cranefly embryos. you have access to this sequence. You are interested in studying the EVE gene
- There is a gene in the fruit fly (Drosophila) called antennepedia. It controls the formation of which structures? What happens when it isIt seems that developmental genetics boils down to a complex network of gene regulation. Try to draw a structure of this network for Drosophila. How many genes do you think are necessary to complete the developmental network for the fruit fly? How many genes do you think are needed for a network to specify one segment? Do you think it is more difficult to identify genes that are involved in the beginning, middle, or end of this network? Suppose you were trying to identify all of the genes needed for development in a chicken. Knowing what you know about Drosophila development, would you first try to identify genes necessary for early development, or would you begin by identifying genes involved in cell differentiation?What is the difference between a maternal-effect gene and a zygotic gene? Of the following genes that play a role in Drosophila development, which are maternal-effect genes and which are zygotic? Explain your answer. A. nanos B. Antp C. bicoid D. lab