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You have cloned the protein coding region of the porin gene from a phage into a bacterial cell for a biotech company who wants to use the porin protein in phage therapy (the porin protein forms pores in the bacterial membrane). However, you notice that the porin gene you have cloned is not translated in the bacterial cells and as a result you cannot isolate the porin protein from those cells. What did you do wrong? O You forgot to include phage RNA polymerase, bacterial RNA polymerases cannot transcribe phage genes O You forgot to include the phage DNA polymerase, the gene cannot be replicated in the bacterial cell O You did not include the Shine and Dalgarno region for this gene

You have cloned the protein coding region of the porin gene from a phage into a bacterial cell for a biotech company who wants to use the porin protein in phage therapy (the porin protein forms pores in the bacterial membrane). However, you notice that the porin gene you have cloned is not translated in the bacterial cells and as a result you cannot isolate the porin protein from those cells. What did you do wrong? O You forgot to include phage RNA polymerase, bacterial RNA polymerases cannot transcribe phage genes O You forgot to include the phage DNA polymerase, the gene cannot be replicated in the bacterial cell O You did not include the Shine and Dalgarno region for this gene

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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You have cloned the protein coding region of the porin gene from a phage into a bacterial cell for a biotech company who wants to use the porin protein in phage therapy (the porin protein forms pores in the bacterial membrane). However, you notice that the porin gene you have cloned is not translated in the bacterial cells and as a result you cannot isolate the porin protein from those cells. What did you do wrong? O You forgot to include the phage RNA polymerase, bacterial RNA polymerases cannot transcribe phage genes O You forgot to include the phage DNA polymerase, the gene cannot be replicated in the bacterial cell O You did not include the Shine and Dalgarno region for this gene
Transcribed Image Text:You have cloned the protein coding region of the porin gene from a phage into a bacterial cell for a biotech company who wants to use the porin protein in phage therapy (the porin protein forms pores in the bacterial membrane). However, you notice that the porin gene you have cloned is not translated in the bacterial cells and as a result you cannot isolate the porin protein from those cells. What did you do wrong? O You forgot to include the phage RNA polymerase, bacterial RNA polymerases cannot transcribe phage genes O You forgot to include the phage DNA polymerase, the gene cannot be replicated in the bacterial cell O You did not include the Shine and Dalgarno region for this gene
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