You have a fluorescent molecule that has an excitation wavelength of 320nm and an emission wavelength of 550nm. (a) What would be the wavelength of photons you would use to excite this fluorophore using two- photon microscopy? (b) Suppose that, unfortunately, the tissue you want to image will absorb any light that has more energy than 3.5*10-¹⁹J. Will two-photon microscopy still work? What's the wavelength of the highest energy of light that will penetrate the tissue? (c) To what part of the EM spectrum do these photons belong? (Give answers for one excitation with one photon, two photons, and the emission wavelength.)

You have a fluorescent molecule that has an excitation wavelength of 320nm and an emission wavelength of 550nm. (a) What would be the wavelength of photons you would use to excite this fluorophore using two- photon microscopy? (b) Suppose that, unfortunately, the tissue you want to image will absorb any light that has more energy than 3.5*10-¹⁹J. Will two-photon microscopy still work? What's the wavelength of the highest energy of light that will penetrate the tissue? (c) To what part of the EM spectrum do these photons belong? (Give answers for one excitation with one photon, two photons, and the emission wavelength.)

College Physics

11th Edition

ISBN:9781305952300

Author:Raymond A. Serway, Chris Vuille

Publisher:Raymond A. Serway, Chris Vuille

Chapter1: Units, Trigonometry. And Vectors

Section: Chapter Questions

Problem 1CQ: Estimate the order of magnitude of the length, in meters, of each of the following; (a) a mouse, (b)...

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Question

You have a fluorescent molecule that has an excitation wavelength of 320nm and an emission
wavelength of 550nm.
(a) What would be the wavelength of photons you would use to excite this fluorophore using two-
photon microscopy?
(b) Suppose that, unfortunately, the tissue you want to image will absorb any light that has more
energy than 3.5*1019J. Will two-photon microscopy still work? What's the wavelength of the
highest energy of light that will penetrate the tissue?
(c) To what part of the EM spectrum do these photons belong? (Give answers for one excitation
with one photon, two photons, and the emission wavelength.)

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