You are about to assay for your LDH using LDH reaction cocktail. Your instructor tells you to dilute your supernatant fraction (Sup) 20x (1/20) for assaying. Describe how do you plan to make this dilution in final volume of 100 I and what pipet(s) do you need.
You are about to assay for your LDH using LDH reaction cocktail. Your instructor tells you to dilute your supernatant fraction (Sup) 20x (1/20) for assaying. Describe how do you plan to make this dilution in final volume of 100 I and what pipet(s) do you need.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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Transcribed Image Text:You are about to assay for your LDH
using LDH reaction cocktail.
Your instructor tells you to dilute your
supernatant fraction (Sup) 20x (1/20) for
assaying.
Describe how do you plan to make this
dilution in final volume of 100
I and what pipet(s) do you need.
Expert Solution
Introduction
Supernatant refers to a clear solution located above the solid residues deposited after certain processes such as precipitation, settling, centrifugation, or crystallization. The solution is a mixture that forms when a solute is dissolved in a solvent. The solvent is a medium that dissolves the substance called the solute.
The process of reducing a solute concentration by adding solvent to a solution is referred to as dilution.
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