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Write out how you would prepare the following solutions: 1. 250 ml of 0.30 M mannitol, 0.025 M phosphate buffer pH 7.2 using mannitol, KH2PO4, and K2HPO4. (Hint: you need to use the Henderson Hasselbalch equation) 2. 100.0 ml of 0.35 M NaCl, 0.02 M Tris buffer pH 7.50 using NaCl and a stock solution of 0.50 M Tris buffer pH7.50. (Hint: you have a concentrated Tris buffer that can be diluted) 3. 50.0 ml of a stock solution of 1.75 M MgCl2. 4. 50.0 ml of a stock solution of 0.50 M KCI.

Write out how you would prepare the following solutions: 1. 250 ml of 0.30 M mannitol, 0.025 M phosphate buffer pH 7.2 using mannitol, KH2PO4, and K2HPO4. (Hint: you need to use the Henderson Hasselbalch equation) 2. 100.0 ml of 0.35 M NaCl, 0.02 M Tris buffer pH 7.50 using NaCl and a stock solution of 0.50 M Tris buffer pH7.50. (Hint: you have a concentrated Tris buffer that can be diluted) 3. 50.0 ml of a stock solution of 1.75 M MgCl2. 4. 50.0 ml of a stock solution of 0.50 M KCI.

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Write out how you would prepare the following solutions: 1. 250 ml of 0.30 M mannitol, 0.025 M phosphate buffer pH 7.2 using mannitol, KH2PO4, and K2HPO4. (Hint: you need to use the Henderson Hasselbalch equation) 2. 100.0 ml of 0.35 M NaCl, 0.02 M Tris buffer pH 7.50 using NaCl and a stock solution of 0.50 M Tris buffer pH7.50. (Hint: you have a concentrated Tris buffer that can be diluted) 3. 50.0 ml of a stock solution of 1.75 M MgCl2. 4. 50.0 ml of a stock solution of 0.50 M KCI.
Transcribed Image Text:Write out how you would prepare the following solutions: 1. 250 ml of 0.30 M mannitol, 0.025 M phosphate buffer pH 7.2 using mannitol, KH2PO4, and K2HPO4. (Hint: you need to use the Henderson Hasselbalch equation) 2. 100.0 ml of 0.35 M NaCl, 0.02 M Tris buffer pH 7.50 using NaCl and a stock solution of 0.50 M Tris buffer pH7.50. (Hint: you have a concentrated Tris buffer that can be diluted) 3. 50.0 ml of a stock solution of 1.75 M MgCl2. 4. 50.0 ml of a stock solution of 0.50 M KCI.
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