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Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Table 1: Restriction digest of plasmid PBR-322 Mass or Tube 1 Tube 2(Eco RI) Tube 4 (Eco RI and Hind III) Component Stock Tube Concentration concentration (control) 3(Hind III) 16 μL 2 µL required sd H20 19 µL 16 uL 2 µL 15 µL 2 µL Reaction 10X 1X buffer Eco RI 1 uL 1 µL 1 uL 20 1 uL 1 µL 1 uL 20 Hind III 1 µL 20 1 uL 20 DNA 1ug Total Pst1 3611 Pvul 3736 Scal 3846 Amp ori PBR322 Vector (4,361bp) EcoR I 4359 Hind III 29 Pvu ll 2066 EcoR V 187 BamH I 375 Tet Sph 1 566 Sal 1 651 BstZ 1 939 Sty I 1369 Figure 1 PBR322 cloning vector
Transcribed Image Text:Table 1: Restriction digest of plasmid PBR-322 Mass or Tube 1 Tube 2(Eco RI) Tube 4 (Eco RI and Hind III) Component Stock Tube Concentration concentration (control) 3(Hind III) 16 μL 2 µL required sd H20 19 µL 16 uL 2 µL 15 µL 2 µL Reaction 10X 1X buffer Eco RI 1 uL 1 µL 1 uL 20 1 uL 1 µL 1 uL 20 Hind III 1 µL 20 1 uL 20 DNA 1ug Total Pst1 3611 Pvul 3736 Scal 3846 Amp ori PBR322 Vector (4,361bp) EcoR I 4359 Hind III 29 Pvu ll 2066 EcoR V 187 BamH I 375 Tet Sph 1 566 Sal 1 651 BstZ 1 939 Sty I 1369 Figure 1 PBR322 cloning vector
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