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Why was it necessary to use phosphoaminophosphohlc (ANP) in the ATP synthase enzyme active site rather than ATP to resolve the molecular structure of the ATP synthase active site by x-ray crystallography?

Why was it necessary to use phosphoaminophosphohlc (ANP) in the ATP synthase enzyme active site rather than ATP to resolve the molecular structure of the ATP synthase active site by x-ray crystallography?

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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Why was it necessary to use phosphoaminophosphonic acid-adenylate ester (ANP) in the ATP synthase enzyme active site rather than ATP to resolve the molecular structure of the ATP synthase active site by x-ray crystallography? ANP Mg2+ E193 T164 x1 E189 B1 ATP cannot be used in a cell free system because it will be readily
Transcribed Image Text:Why was it necessary to use phosphoaminophosphonic acid-adenylate ester (ANP) in the ATP synthase enzyme active site rather than ATP to resolve the molecular structure of the ATP synthase active site by x-ray crystallography? ANP Mg2+ E193 T164 x1 E189 B1 ATP cannot be used in a cell free system because it will be readily
E193 T164 PET89 ATP cannot be used in a cell free system because it will be readily hydrolyzed. ANP has a S-S bond in the purine ring of the nucleotide to aid in crystallography. ATP is twice as large as ANP and will not fit in the ATP synthase active site. O ANP is hydrophobic and ATP is hydrophilic, which makes ANP a better reactant. O ANP is nonhydrolyzable and will trap the enzyme in the desired conformation.
Transcribed Image Text:E193 T164 PET89 ATP cannot be used in a cell free system because it will be readily hydrolyzed. ANP has a S-S bond in the purine ring of the nucleotide to aid in crystallography. ATP is twice as large as ANP and will not fit in the ATP synthase active site. O ANP is hydrophobic and ATP is hydrophilic, which makes ANP a better reactant. O ANP is nonhydrolyzable and will trap the enzyme in the desired conformation.
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