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Why is it no longer considered necessary to determine theN-terminal amino acid of a protein as a separate step?

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter9: Gene Expression And Gene Regulation

Section: Chapter Questions

Problem 14QP: How many kilobases of the DNA strand below will code for the protein product?

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Why is it no longer considered necessary to determine the Nterminal amino acid of a protein as a separate step?
Why is it important to identify the N-terminal residue(s) of a protein?
What useful information might you get if you did determine the N-terminal amino acid as a separate step?
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You are in the process of determining theamino acid sequence of a protein and must reconcile contradictory results. In one trial, you determine a sequence with glycine asthe N-terminal amino acid and asparagine as the C-terminal aminoacid. In another trial, your results indicate phenylalanine as theN-terminal amino acid and alanine as the C-terminal amino acid.How do you reconcile this apparent contradiction?
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How many ways can four different amino acids be arranged in a peptide so that each peptide is unique?
identify the interaction that may be produced between the side chain of amino acid residues found at the N-terminus and the C-terminus of the peptide. List all possible peptides that can be formed from the given peptide (use three character/letter abbreviations for each amino acid, i.e., Ala-Gly-Val). NOTE: Any excess will be deducted to the correct answers. Be careful in evaluating your answers.
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