Why do we need to prepare a standard curve before we can use Beer's Law to determine the concentration of a solution? O We don't have to create a standard curve so long as we choose the appropriate wavelength. O Because the standard curve tells us the absorbance of our sample Because the standard curve is used to determine the extinction coefficient Because the standard curve indicates what wavelength to set the spectrophotometer to
Why do we need to prepare a standard curve before we can use Beer's Law to determine the concentration of a solution? O We don't have to create a standard curve so long as we choose the appropriate wavelength. O Because the standard curve tells us the absorbance of our sample Because the standard curve is used to determine the extinction coefficient Because the standard curve indicates what wavelength to set the spectrophotometer to
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Step 1: Standard Curve
Answer :
In Beer's Law, standard curve is of absorbance vs concentration.
based on the Beer's law equation
A = E*b*c
A = absorbance
b = path length ( generally it is of 1 cm cuvette)
E = extinction coefficient
c = concentration
so if we plot the graph of absorbance vs concentration, it will be a straight line with slope value = extinction coeffecient * path length
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