Why do serial dilution? Does serial dilution make it easier to count bacteria on a spread/streak plate?

Q: You are developing a hypothetical culture medium for the isolation of salmonella Typhimurium. How…

A: Salmonella typhimurium is a gram-negative bacteria and are found in the lumen of intestine. They are…

Q: Why do critical instruments need to be sterile, whereas semicritical instruments need only be free…

A: Laboratory instrument is defined as a general term used for all kinds and types of instruments,…

Q: What is a mixed culture? A contaminated culture?

A: A microbiological culture, or microbial culture, is a technique for increasing microbial creatures…

Q: What is the purpose of flaming an inoculating loop?  How will you know when you have flamed the loop…

A: Hi, since you have posted multiple questions, we will be providing you with answers for the first…

Q: What medium is this? What is it detecting? Why do you see the clear ring around one bacteria (how…

A: Caesin Hydrolysis Test This test is used to determine the ability of the provided bacteria to…

Q: Why is Micrococcus luteus a common contaminate on bacteriological media?

A: The strain is typically connected with human skin, especially from the head, arms and legs;…

Q: What method is best for for isolating a colony on a TSA plate? A. Swab plate B. Straight line…

A: An isolated colony represents a pure source of an organism from which a pure culture can be started.

Q: What is the difference between culture-based technique and culture-independent techniques based on…

A: A microbiological culture, also known as a microbial culture, is a way of multiplying microbial…

Q: What is the difference between complete medium and minimal medium? How are complete media and…

A: Nutritional requirements of bacteria and the culture media are defined by microbiologists. The…

Q: Do we have to do an antibiotic sensitivity test to ensure the result? and what is the type and name…

A: Antibiotic Sensitivity Test:  Antibiotic sensitivity testing, often known as antibiotic…

Q: Why each bacterium must be tested separately?

A: Antibiotics are chemical substances that kill or inhibit or prevent the growth of microbes. They are…

Q: Based on the same two strains of E. coli from the previous question, why do you see growth in the…

A: Ampicillin is a penicillin type of antibiotic substances containing medium So the  strain II can…

Q: Is the isolation plate pigmented? (is their a color for it)? what is colony morphology(single cells…

A: Colony morphology is a method that is used to describe the characteristics of an individual colony…

Q: When bacteria from a throat swab are streaked on blood agar, why is the agar stabbed several times…

A: The initial identification of the streptococcal and enterococcal species is based on their hemolytic…

Q: How can the enrichment culture technique be used medically?

A: Bacteria require appropriate environmental conditions for its growth. Solid or a liquid preparation…

Q: how is the streak plate technique used to isolate and purify psychrotrophic spoilage-causing…

A: Microbiology is an indispensable subject of biological sciences that concerns the study of microbes…

Q: What are the basic differences between the three plating methods? Fill up the following table.…

A: These are the aseptic methods used in laboratories for the growth of micro organisms.

Q: How will you prevent the occurrence of the bacteria present in the Rapid Strep A Test?

A: Answer:  Rapid Strep A Test (Rapid strep test) is a test used to identify the presence of Strep A…

Q: Why is the loop flamed before it is placed in a culture tube? Why is it flamed after completing the…

A: The term sterilization refers to any process that eliminates, kills, removes, or deactivates all…

Q: Both the Endospore Stain Protocol and Mannitol Salt Agar Media are differential. How can this be so?

A: The growth media are nutritious media that are used to grow microbes (bacteria and fungus) in the…

Q: How does plating efficiency affect the number of plaque-forming units? How is plating efficiency…

A: A viral plaque is defined as the visible structure formed on the culture plate of bacteria or any…

Q: Why do you think the continuous culture has higher chances of contamination than batch and fed-batch…

A: Continuous culture may be a set of techniques wont to reproducibly cultivate microorganisms at…

Q: 1. Give 2 systems used at present for the automatic identification of bacteria. 2. Why aseptic…

A: Two methods widely used for bacterial identification in the laboratories are: Chemical assays  PCR…

Q: What is the correct shape of the unknown bacterium growing on your mannitol salt agar

A: Mannitol salt agar also known as MSA is commonly used selective agar in microbiology. This material…

Q: Why did we both stab and streak our blood agar plates when inoculating them with bacteria?

A: Blood agar is a nutrient medium which contain essential nutrients for bacterial growth and blood of…

Q: Would you use an inoculating loop to transfer bacteria to an agar deep tube?

A: Agar deep tube is a glass tube which is filled with solid medium. The medium is solid due to…

Q: Why are traditional tests such as metabolic test not always used to ID clinically important…

A: Traditional methods for identification of microbes include phenotypic tests or biochemical tests…

Q: What is the principle behind invertase test

A: Invertase was an enzyme that hydrolyses the disaccharide sucrose into monosaccharides glucose and…

Q: how does growing and identifying an actual bacterium from skin using culture based methods relate to…

A: Staphylococci are gram-positive cocci about 0.5 to 1.0 μm wide. They grow in groups, in pairs and…

Q: For the following methods of bacterial enumeration does the method determine total count or viable…

A: MPN (i.e. Most probable number), microscopic count , standard plate count, turbidity is type of…

Q: A laboratory technician performed a series of 10 fold serial  dilutions shown below. he plated 0.1…

A: In this question, we are given colony count from different 10 fold dilutions. The volume plated from…

Q: erial slide agglutination technique important in diagnostic procedure?

A: Immunoassays are bioanalytical ways that use the specificity of an antigen-antibody reaction to…

Q: In spread plate method, inoculating loop is used to spread bacteria from the inoculation site. For…

A:

Q: If you did the multiple-tube technique, list one advantage and one disadvantage of each method of…

A: Water from various sources obtained for routine testing were analyzed by Swedish standard methods…

Q: How do you know if you have a ‘countable’ plate when doing a dilution series?   Two plates…

A: The concentration of stock solution can be calculated by first diluting the stock into a suitable…

Q: you are given a mix culture of S aureus, E. coli and P. aeruginosa. besides using the streak plate…

A: The first requirement for physically isolating a bacterium is that it can be cultured in the…

Q: how to find out the test is differential or selective in the microbiology lab test?

A: Many kinds of medias are used in microbial study. Two types of media having similar inferred names…

Q: What is the motivation behind the use of stab slant and surface inoculation

A: Agar slants are prepared by adding the agar media to the test tube and allowing it to cool in a…

Q: Explain why culture based biochemical and genetic testing would not be the ideal method to identify…

A: Microbial culture or microbiological culture is a technique in microbiology in which microorganisms…

Q: What was the concentration of bacteria (CFU/mL) in the original suspensions used for dilutions and…

A: The ideal colony size is found between 30 and 300. The CFU/ml is calculated by: (Colony × Dilution…

Q: Why is it so important to develop tests to ID bacteria

A: Answer: BACTERIA : These are the unicellular microorganisms, which are prokaryotic in nature.…

Q: James inoculated a species of bacteria into KIA media and realized that it has a yellow butt and a…

A: Introduction KIA or the Kilger Iron Agar is a differential media that helps in distinguishing the…

Q: 1- Why was the T4 and E.coli mixed together in top agar and then poured on the plate instead of just…

A: T4 bacteriophage and E. coli mixture using in plaque assay :-

Q: Why are aseptic techniques implemented during inoculation?

A: Aseptic means without contamination and Inoculation means transfer of a bacterial sample onto a…

Q: A microbiologist sets up a serial dilution to analyze bacteria in raw milk.   Tube 1 – 0.1 mL milk…

A: Dilution is a phenomenon in which final solution are prepared having concentration comparatively…

Q: What is the purpose of using the streak plate method?

A: Solution-  Introduction -  Streak plate technique is used  for the isolation into a pure culture of…

Q: Are there technologies that can help make Bacterial Culture and Sensitivity done faster?

A: Microbial culture refers to the culture or growing the bacteria in the laboratories on liquid and…

Q: What is the goal of isolation streak plate technique? Why is microbial considered a pure culture?

A: Streaking : Streaking for isolation on an agar plate involves the successive dilution of organisms…

Microbial Growth

The development of microbial growth is influenced by various natural conditions (both physical and synthetic) like pH, temperature, saltiness, gas focus (O2, CO2, and H2), nutrients, water action, and assimilation. Knowing the distinctive physical and chemical factors that influence the development and endurance of microbial growth in the climate will assist with reinforcing endeavors outfitted towards their control. These lines lessen or diminish their harmful impacts on humankind, creatures, and the climate.