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Which statement below about Bradford assay is wrong? O Coomassie Brilliant Blue dye is used to bind certain proteins specifically. BSA is used to make standard curve. Unknown protein concentration can be calculated once BSA standard curve is generated. O Similar to A280, Bradford assay is used to determine total protein concentration.

Which statement below about Bradford assay is wrong? O Coomassie Brilliant Blue dye is used to bind certain proteins specifically. BSA is used to make standard curve. Unknown protein concentration can be calculated once BSA standard curve is generated. O Similar to A280, Bradford assay is used to determine total protein concentration.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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Which statement below about Bradford assay is wrong? O Coomassie Brilliant Blue dye is used to bind certain proteins specifically. BSA is used to make standard curve. Unknown protein concentration can be calculated once BSA standard curve is generated. O Similar to A280, Bradford assay is used to determine total protein concentration.
Transcribed Image Text:Which statement below about Bradford assay is wrong? O Coomassie Brilliant Blue dye is used to bind certain proteins specifically. BSA is used to make standard curve. Unknown protein concentration can be calculated once BSA standard curve is generated. O Similar to A280, Bradford assay is used to determine total protein concentration.
Which statements about common buffer systems use in biochemistry labs is incorrect? O In the pKa region of a buffer solution, pH changes greatly as H+ or OH- is added. O Buffer systems resist large pH changes. O Common buffer systems contain conjugate acid and base. O pH of any buffer solutions does change.
Transcribed Image Text:Which statements about common buffer systems use in biochemistry labs is incorrect? O In the pKa region of a buffer solution, pH changes greatly as H+ or OH- is added. O Buffer systems resist large pH changes. O Common buffer systems contain conjugate acid and base. O pH of any buffer solutions does change.
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