Which samples contain the blood of the father of the child?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Which samples contain the blood of the father of the child?

**Part A: Forensic Science—A Paternity Case**

Assume that you are a molecular biologist involved in analyzing a paternity dispute. In order to determine with accuracy which of the men listed contributed genetic material to the child, you took blood samples from the mother, child, and each potential father. Then you ran the samples on an agarose gel electrophoresis, adding an appropriate restriction enzyme to each of the samples. Results from the restriction enzyme digest are as follows:

**Gel Diagram Explanation:**

The diagram shows the results of an agarose gel electrophoresis for five samples, with vertical lanes labeled 1 to 5. Each lane contains horizontal bands that represent DNA fragments of different lengths.

- **Well 1: Mother**
- **Well 2: Child**
- **Well 3: Father 1**
- **Well 4: Father 2**
- **Well 5: Father 3**

**Interpretation:**

- **Well 1 (Mother):** Contains a unique pattern of DNA bands.
- **Well 2 (Child):** Has bands that match some from the mother and must match those from the biological father.
- **Well 3 (Father 1), Well 4 (Father 2), and Well 5 (Father 3):** Each lane shows different band patterns. Comparison is required to see which father's bands match those of the child.

By comparing the child’s DNA bands with those of the mother and the potential fathers, you can determine the child’s biological father. Matching bands between the child and a potential father that are not found in the mother indicate paternity.
Transcribed Image Text:**Part A: Forensic Science—A Paternity Case** Assume that you are a molecular biologist involved in analyzing a paternity dispute. In order to determine with accuracy which of the men listed contributed genetic material to the child, you took blood samples from the mother, child, and each potential father. Then you ran the samples on an agarose gel electrophoresis, adding an appropriate restriction enzyme to each of the samples. Results from the restriction enzyme digest are as follows: **Gel Diagram Explanation:** The diagram shows the results of an agarose gel electrophoresis for five samples, with vertical lanes labeled 1 to 5. Each lane contains horizontal bands that represent DNA fragments of different lengths. - **Well 1: Mother** - **Well 2: Child** - **Well 3: Father 1** - **Well 4: Father 2** - **Well 5: Father 3** **Interpretation:** - **Well 1 (Mother):** Contains a unique pattern of DNA bands. - **Well 2 (Child):** Has bands that match some from the mother and must match those from the biological father. - **Well 3 (Father 1), Well 4 (Father 2), and Well 5 (Father 3):** Each lane shows different band patterns. Comparison is required to see which father's bands match those of the child. By comparing the child’s DNA bands with those of the mother and the potential fathers, you can determine the child’s biological father. Matching bands between the child and a potential father that are not found in the mother indicate paternity.
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