Which of the two carbon sources, glucose or acetate, is more advantageous for the cultivation of E.coli bacteria? Why?
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Which of the two carbon sources, glucose or acetate, is more advantageous for the cultivation of E.coli bacteria? Why?
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- Clostridium tetani is a common soil bacterium and causes tetanus. Would you expect C. tetani to possess the enzyme catalase? Explain.The protease used in this procedure functions best at 50°C. Would you expect this enzyme to be isolated from Escherichia coli bacteria? Explain your answer. Hint: Where does E. coli live?A 3.0 L batch bioreactor is used to grow E. coli bacteria, which have an initial concentration of 12.5 g cells/L. The growth medium contains two carbon sources, which causes diauxic growth, with each carbon source limiting cell growth. Sufficient supplies of oxygen, ammonium nitrate (NH4NO3) as a nitrogen source and all other macro and micronutrients are available. The bacteria prefer to grow on glucose (C6H12O6, supplied at 80 g/L), so it is consumed first, followed by glycerol (C3H803, supplied at 68 g/L). Since the E. coli were already growing on glucose, the lag time associated with glucose consumption is only 10 minutes. The lag time associated with glycerol consumption is 35 minutes. Further information: The chemical formula for E. coli in this bioreactor is CH1.666N0.2000.57. The specific growth rate on glucose is 0.41 h-1, and on glycerol is 0.32 h-1?The yield of cells on glucose is 0.48 g cells/g glucose, and yield on glycerol is 0.39 g cells/g glycerol. You may assume that…
- Yoghurt starter culture is a mixed population of S. thermophilus and L. bulgaricus, both competing for the same substrate, lactose. Is it possible for the two cultures to continue to co-exist?A 3.0 L batch bioreactor is used to grow E. coli bacteria, which have an initial concentration of 12.5 g cells/L. The growth medium contains two carbon sources, which causes diauxic growth, with each carbon source limiting cell growth. Sufficient supplies of oxygen, ammonium nitrate (NH4NO3) as a nitrogen source and all other macro and micronutrients are available. The bacteria prefer to grow on glucose (C6H12O6, supplied at 80 g/L), so it is consumed first, followed by glycerol (C3H8O3, supplied at 68 g/L). Since the E. coli were already growing on glucose, the lag time associated with glucose consumption is only 10 minutes. The lag time associated with glycerol consumption is 35 minutes. Further information: The chemical formula for E. coli in this bioreactor is CH1.666N0.20O0.57. The specific growth rate on glucose is 0.41 h-1 , and on glycerol is 0.32 h-1 . The yield of cells on glucose is 0.48 g cells/g glucose, and yield on glycerol is 0.39 g cells/g glycerol. You may assume that…You are characterizing a newly discovered organism . You have noticed that the organism grows on two carbon sources - sucrose and mannitol . Whenever you add both sugars to the medium , sucrose will be completely metabolized before mannitol . a ) What is this growth pattern called ? b ) Describe a possible growth curve of sequential metabolism of the sugars .
- If the result of an unknown bacteria is “poor growth” and “red growth,” can this result be interpreted as “organism does not ferment mannitol?” Explain why or why not. If the result of an unknown bacteria is “good growth” and “yellow growth,” is the unknown bacteria more likely Staphylococcus aureus or Staphylococcus epidermidis?I am having trouble figuring out the variables for this problem. I know that the agar is an independent variable but I am not sure if the E. Coli is also an independent variable . Thank you .A researcher is trying to develop a media to isolate a strain of E.coli from a patient’s stomach. The research gives you an intestinal sample from the patient and she assurers you this sample contains the bacterial strain. Previous research shows that this strain of E.coli requires calpain to survive, which is a common enzyme found in beef. Using this information about calpain, how would you develop media to isolate this bacterial strain of E.coli?
- Sydney Brenner isolated Salmonella typhimurium mutants that were implicated in the biosynthesis of tryptophan and would not grow on minimal medium. When these bacterial mutants were tested on minimal medium to which one of four compounds (indole glycerol phosphate, indole, anthranilic acid, and tryptophan) had been added, the growth responses shown in the following table were obtained. Mutant Minimal medium Anthranilic Indole glycerol acid Indole Tryptoph phosphate trp-1 trp-2 trp-3 trp-4 trp-6 trp-7 trp-8 trp-9 trp-10 trp-11 - Give the order of indole glycerol phosphate, indole, anthranilic acid, and tryptophan in a biochemical pathway leading to the synthesis of tryptophan. Indicate which step in the pathway is affected by each of the mutations. + 1 + 1 IIWhen both glucose and lactose are present in the media in which E. coli is growing, which is the preferred carbon source? a)Lactose b)Both Glucose and Lactose c)Xylose d)GlucoseWhen would you expect to see the E. coli produce the enzyme beta galactosidase if the growth media contained only glucose as an energy source?