Which of the following test can compare microbial communities over a period of time? a Protein markers estimation. b Fluorescent in situ hybridization. c Proteomics d DNA finger printing.
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Which of the following test can compare microbial communities over a period of time?
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- MR test result is on the left, VP test result is on the right.a. Please interpret these results. b. A fellow researcher and you are sure your bacterial sample is either E. coli or E. aerogenes. Based upon the work we have done in lab which methods will help you determine which bacteria it is? Please explain why, the results you would want to observe and provide analysis of those results.In fundamental skills for the microbiology laboratory, examine the quadrant streak and T- streak plates. Have your lab partner write a critique of your isolation technique in the space below. The following should be addressed: was isolation produced on one or both plates? On the quadrant streak, we're the first three streaks near the edges of the plate? On both plates, did any streaks intersect streaks they should not have? Was the whole surface of the agar used? Was the agar cut by the loop? Page 41(microbiology lab manual)explain how the following genotypic andphenotypic methods of microbe identification is done. Use diagrams or schematic representations to explain yourwork. 1. Pulsed-Field Gel Electrophoresis (PFGE)2. Whole Genome Sequencing (WGS)
- What can be the errors and limitations of Gel Electrophoresis practical? Explain in very much detail and also provide some better suggestions.The techniques for identifying unknown bacteria can be summarized in three key steps, explain in your own words how to: Staining the unknown for initial characterization by microscopy. Using a dichotomous key strategy to systematically rule out other organisms. Testing the organism for key biochemical traits.explain how the following genotypic andphenotypic methods of microbe identification is done. Use diagrams or schematic representations to explain yourwork. 1.Gram Staining2.Biochemical Reactions: Multiple Tube Fermentation Technique3.(Biochemical Reactions: IMViC Test
- A sample of well water is tested for its bacterial content in a plate count assay. A one-milliliter sample of the water is diluted in a 1:10 dilution series. One milliliter of the fourth dilution tube is plated in a pour plate. After incubation, the plate has 83 colonies. How many bacteria did the original sample contain?Which of the following is a reason to run simultaneous tests on known positive and negative controls when identifying an unknown microbe using biochemical tests? The controls maintain the differential qualities of the test medium The controls can show if there was contamination of the tubes with another species The controls will ensure that the unknown microbe multiplies on the medium The controls can show if there was a recent mutation in your unknown microbePerforming a bacterial isolation by streaking, using sterile (aseptic) technique, consider the expected outcome on the streak plate after the following: Two organisms were in the original sample. Organism G was present at twice the abundance of Organism H (so 2/3 of the original sample). The streak plate was generated using 4 areas (quadrants)- A, B, C & D. Area A was inoculated with a loop containìng 3 mìllion organisms from the original culture, which was mixed very well and considered to be homogeneous. Areas B, C & D were streaked from the previous area with a sterilized loop, with each streak transferring 2% of the organisms from the previous area (assume the relative abundance of the two organisms was maintained). What is the expected outcome in Area on this streak plate? 2d
- How could you utilize fluorescence quenching in the different analytical application? Please answer shorty at your own words. Answer should be specific (4-5 lines) .You are concerned that the level of lead in drinking water has reached potentially harmful levels in certain regions of the country. How would you go about testing what concentrations of lead would be safe for consumption? Include at least one approach from the following 3 categories: in vitro assay, cell-based test, and, toxicogenomic approach.What is the purpose of diluting a culture by making "quadrant streaks" (or "streak plating") bacteria, and what can we use them for? Check all that apply A. To observe colony morphology/color B. To isolate viral plaques C. testing growth conditions or treatments (such as media, antibiotics, incubation temperature etc.,) D. To isolate a clonal population of bacteria from the rest of the culture E. For subculturing (transferring to new media so we can grow more bacteria, storing the plate in a fridge for repeated use so the bacteria don't die quickly) F. Accurate quantification (to calculate CFU/mL) G. To know what strain of bacteria you're working with, since you can tell based on how it looks on the plate. H. To lower risk of contamination/identify contamination