Which of the following statements best describes your observations? Larger inner column diameters require higher flow rates and larger volumes of mobile phase to reach a good velocity, therefore the 4.6 mm column is better equipped at managing an increase of injection volume, whereas the 2.3 mm column becomes overloaded, and resolution suffers Increasing the injection volume overloads both columns, equally, therefore the resolution becomes worse, equally, for both columns Larger inner column diameters result in a decrease of the terms in the van Deemter equation; therefore the 4.6 mm column has less band broadening, and its resolution is better than the 2.3 mm column Smaller inner column diameters have less longitudinal diffusion, therefore increasing the injection volume has a worse impact on the resolution of the 4.6 mm column
Which of the following statements best describes your observations? Larger inner column diameters require higher flow rates and larger volumes of mobile phase to reach a good velocity, therefore the 4.6 mm column is better equipped at managing an increase of injection volume, whereas the 2.3 mm column becomes overloaded, and resolution suffers Increasing the injection volume overloads both columns, equally, therefore the resolution becomes worse, equally, for both columns Larger inner column diameters result in a decrease of the terms in the van Deemter equation; therefore the 4.6 mm column has less band broadening, and its resolution is better than the 2.3 mm column Smaller inner column diameters have less longitudinal diffusion, therefore increasing the injection volume has a worse impact on the resolution of the 4.6 mm column
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Transcribed Image Text:Which of the following statements best describes your observations?
○ Larger inner column diameters require higher flow rates and larger volumes of mobile phase to reach
a good velocity, therefore the 4.6 mm column is better equipped at managing an increase of injection
volume, whereas the 2.3 mm column becomes overloaded, and resolution suffers
Increasing the injection volume overloads both columns, equally, therefore the resolution becomes
worse, equally, for both columns
○ Larger inner column diameters result in a decrease of the terms in the van Deemter equation;
therefore the 4.6 mm column has less band broadening, and its resolution is better than the 2.3 mm
column
Smaller inner column diameters have less longitudinal diffusion, therefore increasing the injection
volume has a worse impact on the resolution of the 4.6 mm column
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