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Which of the following protein-miícrosome arrangements below fits with the data above? d. b. O O O 0

Which of the following protein-miícrosome arrangements below fits with the data above? d. b. O O O 0

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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**Question:** Which of the following protein-microsome arrangements below fits with the data above? **Options:** - a. - b. - c. - d. **Diagram Descriptions:** - **Diagram a:** A circle labeled with "C" and "N" near the center, connected with a blue squiggly line representing a protein layout. - **Diagram b:** A circle with "N" near the top and "C" near the right, connected with a longer blue line that loops once within the circle. - **Diagram c:** A circle with "C" near the top and "N" near the center, connected with a blue line that loops once but is oriented differently than in Diagram b. - **Diagram d:** A circle with "N" near the top left and "C" near the right, connected with a complex blue line that loops twice within the circle.
Transcribed Image Text:**Question:** Which of the following protein-microsome arrangements below fits with the data above? **Options:** - a. - b. - c. - d. **Diagram Descriptions:** - **Diagram a:** A circle labeled with "C" and "N" near the center, connected with a blue squiggly line representing a protein layout. - **Diagram b:** A circle with "N" near the top and "C" near the right, connected with a longer blue line that loops once within the circle. - **Diagram c:** A circle with "C" near the top and "N" near the center, connected with a blue line that loops once but is oriented differently than in Diagram b. - **Diagram d:** A circle with "N" near the top left and "C" near the right, connected with a complex blue line that loops twice within the circle.
### Experimental Analysis of Protein Treatment in Microsomes This image presents a comparative analysis of the effects of different treatments on proteins in the presence and absence of microsomes. The results are visualized using a protein gel electrophoresis. #### Treatments Analyzed - **Protease** - **Detergent** - **Endoglycosidase H (endo H)** Each treatment condition is indicated by a combination of plus (+) and minus (−) signs, representing the presence and absence of the treatment, respectively. #### Experiment Conditions 1. **Microsomes Absent:** - **Lane 1:** No treatment (-, -, -) - **Lane 2:** Protease only (+, -, -) - **Lane 3:** Protease and detergent (+, +, -) - **Lane 4:** Endo H only (-, -, +) 2. **Microsomes Present:** - **Lane 5:** No treatment (-, -, -) - **Lane 6:** Protease only (+, -, -) - **Lane 7:** Protease and detergent (+, +, -) - **Lane 8:** Endo H only (-, -, +) #### Observations - **Lanes 1 and 5:** Show bands indicating the presence of intact proteins. - **Lanes 2 and 6:** Display changes due to protease treatment, suggesting degradation or modification. - **Lanes 3 and 7:** Highlight the combined effect of protease and detergent, showing further variations. - **Lanes 4 and 8:** Endo H treatment causes visible shifts in banding patterns, indicative of changes in glycosylation states. This experiment illustrates the distinct impact of enzymatic and chemical treatments on protein structures both in isolation and within a microsomal environment. The presence and alteration of bands serve as key indicators of protein processing and modification.
Transcribed Image Text:### Experimental Analysis of Protein Treatment in Microsomes This image presents a comparative analysis of the effects of different treatments on proteins in the presence and absence of microsomes. The results are visualized using a protein gel electrophoresis. #### Treatments Analyzed - **Protease** - **Detergent** - **Endoglycosidase H (endo H)** Each treatment condition is indicated by a combination of plus (+) and minus (−) signs, representing the presence and absence of the treatment, respectively. #### Experiment Conditions 1. **Microsomes Absent:** - **Lane 1:** No treatment (-, -, -) - **Lane 2:** Protease only (+, -, -) - **Lane 3:** Protease and detergent (+, +, -) - **Lane 4:** Endo H only (-, -, +) 2. **Microsomes Present:** - **Lane 5:** No treatment (-, -, -) - **Lane 6:** Protease only (+, -, -) - **Lane 7:** Protease and detergent (+, +, -) - **Lane 8:** Endo H only (-, -, +) #### Observations - **Lanes 1 and 5:** Show bands indicating the presence of intact proteins. - **Lanes 2 and 6:** Display changes due to protease treatment, suggesting degradation or modification. - **Lanes 3 and 7:** Highlight the combined effect of protease and detergent, showing further variations. - **Lanes 4 and 8:** Endo H treatment causes visible shifts in banding patterns, indicative of changes in glycosylation states. This experiment illustrates the distinct impact of enzymatic and chemical treatments on protein structures both in isolation and within a microsomal environment. The presence and alteration of bands serve as key indicators of protein processing and modification.
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