Which of the following is true regarding aceto-orcein staining? The active dyes in this strain are small, extremely hydrophobic anionic dyes. Orcein can be extracted from three different species of flower. Staining occurs via hydrophobic bonding and van der Waals attractions. Is ineffective for staining as DNA is soluble in acetic acids.

Q: Both crystal violet and safranin are basic stains and may be used to do simple stains on Gram…

A: Gram staining is the common procedure followed to distinguish the bacteria based on their cell wall.…

Q: draw the teeth smear with methylene blue on positive simple staining in 1000x objective

A: The teeth smear can be obtained to detect the presence of microbes present in the oral cavity and…

Q: What do you think is/are the characteristic of sulfamethoxazole (antibiotic) that allows it to…

A: This is the structure of Sulfamethoxazole.

Q: Why are basic dyes more effective for bacterial staining than acidic dyes?

A: Staining is a technique used in slide preparation to colour the specimen.      With light microscopy…

Q: Which of the following is true regarding aceto-orcein staining?

A: Aceto-orcein dye is a brownish-red and crystalline dye. It consists of a variety of phenaxazones,…

Q: Why is the wavelength of the light that is released in spectrophotometric analysis longer than the…

A: Some molecules absorb light at a particular wavelength and emit light in higher wavelength and this…

Q: Which of the following statements is FALSE?   Question options:   stains increase the contrast of…

A: 3rd statement is false i.e the chromophore of an acidic dye is positively charged.

Q: What are the principle and basic concepts of DIFFERENTIAL staining?

A: The simple dye is used in the technique of simple staining to highlight only the specific structures…

Q: What is the purpose of acid-fast staining? What information can you gain from this procedure? Please…

A: The acid-fast stain is used to identify acid-fast organisms. Acid-fast organisms posess nearly…

Q: gel electrophoresis

A: Gel electrophoresis is a procedure that used to separate biological molecules by size. The…

Q: What is the principle of Giemsa staining and  components of Giemsa stain?

A: The cells are the basic structural and fundamental unit of the living system. It consists of…

Q: Alkaline phosphatase conjugated to the anti-DIG antibody recognizes the Dig-labeled probe. The…

A: Introduction  Alkaline phosphatase (AP) is an enzyme that can catalyze the hydrolysis of various…

Q: Identify the following on the gel electrophoresis 2-leg ladder 3 Kb 1022 bp 1022 bp 880 bp 765 hp…

A: Biotechnology is the use of our understanding of biological processes to develop useful applications…

Q: When it comes to simple stains, why is time so important? And what is the purpose of rinsing the…

A: Microbiology is a hugely important field in both basic and applied research. New and fascinating…

Q: Select all of the following that represent examples of positive stains. A) nigrosin B) methylene…

A: Staining is a technique usually employed to enhance the contrast of specimens in microscopy. Many…

Q: How are negative stains prepared?Name two applications for which this type of stain especially…

A: Negative staining is defined as a method of demonstrating the type of small objects (like bacteria)…

Q: If you want a better resolution, would you have increased or decreased the agarose percentage in the…

A: The composition of agarose gels has an effect on the migration rates of DNA molecules. As the…

Q: What happens to the extent of inversion when the sugar was heated for sixty minutes instead of…

A: Dextrorotatory - Compound with positive optical rotation. Levorotatory - Compound with negative…

Q: At what wavelengths should the absorbances be read to examine the purity of a DNA sample with a…

A: A spectrophotometer is a tool that measures the number of photons (light energy) absorbed after…

Q: Create paragraphs to differentiate among the following terms: positive stain, negative stain, simple…

A: An organic molecule with a benzene ring, a chromophore, and an auxochrome group is known as a…

Q: What is FRAP (Fluorescence Recovery After Photobleaching)? Draw a graph of fluorescence versus time…

A: FRAP or Fluorescence Recovery After Photobleaching It is a method that was developed in 1976 by…

Q: Anthocyanins are another type of plant pigment that are purple or red. Say you run a chromatography…

A: Chromatography is a method of separation in which the analyte is mixed with a liquid or gaseous…

Q: Staining procedure Dye (Basic or acidic) Charge of the chromophore/ colored ion Charge of the target…

A: Staining methods are used to improve and enhance the sample contrast for it to be studied under a…

Q: Why is nigrosin-water- bacteria puddle need to be mixed in a Simple/Negative staining

A: Staining is a technique used in slide preparation to color the specimen. With light microscopy,…

Q: Methylene blue can be prepared as a basic stain or an acidic stain. How would the pH of the stain…

A: 1.Since the bacteria are negatively charged, a positive charge was needed to cause staining. The…

Q: list the major steps, in sequence needed to perform the schaeffer-fulton and crystal violet…

A: Endospores are structures produced within certain bacterial cells that allow them to survive harsh…

Q: Which among the following demonstrates the difference between Native Polyacrylamide Gel…

A: PAGE or polyacrylamide gel electrophoresis is a separation technique under the influence of…

Q: What staining technique is used here? What is the structure in green? What is the structure in…

A: When spores are stained using special technique such as schaeffer Fulton method  That used to…

Q: What is the main advantage of fluorescence polarization? Sensitivity of fluorescent probes Low cost…

A: FP is an analytical technique which provides information on orientation and mobility of…

Q: In extraction of albumin in egg white using 100-ml water, what does albumin look like? Also, what do…

A: In extraction of albumin in egg white and placed in water some reactions are occur..

Q: What are the characteristics of an acidic dye? Of a basic dye? Indicate whether methylene blue is an…

A: Dyes are colored compounds that are used as coloring agents in laboratories, textile industry, etc.…

Q: You and a partner conduct the acid-fast staining using the same two bacteria. You know that there…

A: Acid-fast staining is a differential staining technique that proves to be an important diagnostic…

Q: Differentiate the “hot method” and “cold method” of acid-fast staining

A: Introduction Staining is a technique for enhancing contrast in material, usually on a microscopic…

Q: Select all of the following that represent examples of positive stains. Check All That Apply…

A: Staining is the common laboratory procedure which can be specific and general according to the use.…

Q: Briefly explain the difference between Wright Staining and H&E Staining (why use one over the other)

A: Staining is defined as a technique ,which is utilized for increasing the contrast in biological…

Q: How could you utilize fluorescence quenching in the different analytical application? Please answer…

A: Fluorescence quenching is a method that diminishes the fluorescence force of an sample. A number of…

Q: Students subjected three samples of five different molecules to gel electrophoresis as shown in…

A: Gel electrophoresis is a technique in molecular biology in which different samples (DNA, RNA or…

Q: How does electrophoresis separate dye pigments? What charge is carried by the pigments in this…

A: Electrophoresis d the process of separation of DNA/ protein molecules on the basis of size. The DNA…