Which of the following involves a triploid? (Select all that apply OR select "none of the above" if no choices apply.) 2N +1 +2 3N -2 +2 O4N -2 -1 5N -2 -2 none of the above
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A:
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- Below is an EMSA showing four different reactions, A-D. In each tube there is some combination of labelled DNA probe, Protein X (the protein you are studying), and an antibody for Protein X. Identify which combination of components are found in each of the four reactions and explain how you determined that based on the molecular interactions being studied and your knowledge of gel electrophoresis. It is possible that multiple lanes have the same component(s). A B C D EMSAIf the primers you purchased possessed the following information. 5'-GGA AAC AGC TAT GAC CAT G-3' Calculate the melting temperature of this primer and estimate the annealing temperature of this primer.At 1x concentration, the orange G dye has 5% glycerol. The dense glycerol will help keep your DNA in your samples from floating out of the wells upon loading the gel. What is the concentration of glycerol in the 6x orange G stock solution? Be sure to show your calculations.
- You will use a Calf Thymus DNA standard stock solution (1 mg/ml) to prepare a standard curve. Calculate volume of stock solution in ul, needed to prepare 10 ug/ml dilution (final volume 1.0 ml).A vial of Doxorubicin reads 0•5g per vial. Instructions say to reconstitute each 12mg with 2•5ml of NS. How many ml of NS will be needed to reconstitute the vial of the recommended concentration? please show workingA series of bovine serum albumin (BSA) was prepared and 1 mL of each solution was subjected to a Bradford assay. The increase in absorbance at 595 nm relative to the concentration of protein was plotted as shown below. Save 1.0- 0.8- 0.6- 0.4- 0.2- 0.04 0.0 0.5 1.0 2.0 2.5 1.5 BSA concentration (mg/mL) a) Using the graph, calculate the original concentration of protein present in a mixture of haemoglobin and methylene blue (diluted 1:50), which gave an absorbance of 0.20 at a wavelength of 595 nm using the Bradford assay. Show all workings. Absorbance (O.D.)
- A plant isolate was subjected to qualitative tests to determine the presence of macromolecules. Below is a summary of results obtained: Biuret Ninhydrin Schiff's Test for Test for test test test Deoxyribose Phosphat Violet Violet Pink Blue Yellow solution solution solution solution precipitat Conclusion: Chromosomal DNA was possibly extracted from the plant. O True O FalseComplete the table below by adding (+) or (-) if eahc sample (amino acid or protein) below will test positive or negative, respectively. sample BIURET NINHYDRIN XANTHOPROTEIC MILLON-NASSE SAKAGUCHI LEAD ACETATE PAULY HOSKIN-COLE G W H Y C R HAIR GELATIN CASEIN PEPTONE Identify the amino acid that will give the following data: BIURET NINHYDRIN XANTHOPROTEIC MILLON-NASSE SAKAGUCHI LEAD ACETATE PAULY HOPSKIN-COLE - + + + - - + - Name of amino acid: structurte of the amino acid:what is the full name of the TISSUSE TYPE at the end of the arrow for letter F? F1) Does this look like any other lettered structure on this photomicrograph? If yes, which one and how are they similar.
- Owing to their structural similarity, separation of mixtures of organic compounds (such as the isomers 1-t- butyl-, 3-t-butyl-, and 4-t-butylcyclohexene) often proves difficult. Silica gel chromatography, however, can be used with some success provided that sufficient silica gel is used to provide optimal separation. Consider, for example, the TLC plate pictured at right after developing and UV visualization where prior to elution, lanes A and B were spotted with 1-t-butylcyclohexene and lanes B and C spotted with 4-t- butylcyclohexene. Which of the following must be true? In the space provided, indicate the CORRECT answers (as 1, 2, and/or 3) then explain your reasoning for all three statements. 1. 1-t-butylcyclohexene elutes faster than 4-t-butylcyclohexene. 2. The R; for both 1-t-butylcyclohexene and 4-t-butylcyclohexene are equal. 3. 4-t-butylcyclohexene is less polar than 1-t-butylcyclohexenePlease provide a separation solution of combining two types of chromatography to isolate the protein A from the mixture containing protein A, B, C and D (shown as below). And then explain the related mechanisms in details. Protein A: Met-Leu-Leu-Leu-Leu-Val-Val-Val-lle-lle-lle-Leu-Leu-Leu-Leu- Protein B: Met-Asp-Glu-Glu-Glu-Glu-Asp-Asp-Asp-Glu-Glu-Asp-Asp-Glu Protein C: Met-Leu-Leu-Leu-Leu-Val-Val-Val-lle-lle-lle-Leu-Leu-Leu-Leu-Val-Val-Val- Ile-lle-lle-Leu-Leu- Leu-Leu-Val-Val-Val-lle-lle-lle-Leu-Leu-Leu-Leu-Val-Val-Val-lle-lle- lle-Leu-Leu-Leu-Leu-Val-Val-Val-Ile- Protein D: Met-Asp-Glu--Glu-Glu-Glu-Asp-Asp-Asp-Glu-Glu-Asp-Asp-Glu-Glu-Asp- Glu-Asp-Glu-Glu-Asp-Asp-Glu-Glu-Glu-Glu-Asp-Asp-Asp-Glu-Glu-Asp-Asp-Glu-Glu- Asp-Glu-Asp-Glu-Glu-Asp-Glu-Glu-AspElectrophoresis is performed at PH 6.8 on a mixture of mutated hemoglobin that differ from normal haemoglobin (Hb) only by the substitution of one amino acid- Hb X: Val replaced par Glu - Hb Y: Asp replaced by Leu - Hb Z: Glu replaced by Lys What will be the order of migration between cathode and anode of these mutated Hb compared to normal Hb? Justify your answer.