When a disinfectant or antiseptic's effectiveness is expressed as a phenol coefficient, a number ____ 1.0 means the chemical agent is more effective than phenol. less than greater than equal
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- Detergents are______.What is the minimal concentration (MIC) for disinfectant number 1? Just write the corresponding number including the decimal as written. ug/mL. Disinfectant 1 2 3 4 concentration 0.1 ug/mL +++ ++++ ++ +++ 0.2 ug/mL ++ ++ + ++ 0.5 ug/mL + + 1.0 ug/mL 5.0 ug/mL subculture 1 2 3 4 0.1 ug/mL ++++ ++++ ++++ ++++ 0.2 ug/mL ++++ ++++ ++++ ++++ 0.5 ug/mL ++++ ++++ ++++ 1.0 ug/mL ++++ 5.0 ug/mL Submit responseIn sterilization, which among the supplies, instruments, glassware, etc. under the list of materials can be sterilized using either or both equipment below? List them down. For "autoclaving" For Dry heat oven Can be sterilized only sterilization with either Materials: 200-ml Erlenmeyer flask Stove 500-ml Erlenmeyer flask Autoclave 10-ml graduated cylinder Analytical balance 100-ml graduated cylinder pH meter Spatula Stirring rod 100-ml beaker Test tubes Distilled water Petri dish Stirring rod Alcohol lamp Glass dropper
- In a(n) _________ test, the effectiveness of a microbial control agent is compared to that of carbolic acid. Question options: use-dilution in-use Kelsey-Sykes capacity phenol coefficient Entner-Doudoroff algebraSalmonella-Shigella (SS) agar plate results (kitchen sink drain and kitchen dish cloth) Using the following information about SS agar and the image below, describe and identify the growth on the SS agar plate in the table on page 6. SS agar plate - results of kitchen sink drain and kitchen dish cloth (plate below) Salmonella-Shigella (SS) agar: Salmonella-Shigella agar (SS agar) is a selective and differential medium utilized for the selection and characterization pathogenic enteric bacteria. SS agar contains lactose, bile salts, ferric citrate, and neutral red. Kitchen The bile salts select for Gram-negative bacteria, while inhibiting Gram-positive bacteria. sink drain The other components differentiate among Gram-negative bacteria. Coliform bacteria such as E. coli will ferment the lactose in the media, resulting in bacterial growth with a pink color. (They do not produce any hydrogen sulfide.) Members of the genus Salmonella will not ferment lactose, but do product hydrogen sulfide…5 1 2 6 Testing Anti-Microbial Agents: Disk-Diffusion. Lab Report Fill out the zones of inhibition table with your data: Agent Tested Cleansing Spray 3 4 Foaming Handwash Hand Sanitizer Lysol spray Detergent Desinfecting zone of inhibition: (mm) E. coli 10mm 15 mm 20mm 0mm 9mm P. aeruginosa 8 mm 35mm 17mm 15mm 0 mm 1mm Hydrogen Peroxide Here, write a few observations about your experimental results. 25mm S. aureus 25mm 20mm 25mm 12mm 8mm 42mm What factors (at least 2) other than the identity of the agent being tested can influence the size of the zone of inhibition? NOT Were any colonies observed within the zone of inhibition? Even if you didn't have any, explain why you might see colonies in the zone of inhibition. Be specific about how that might happen? What follow up questions or conclusions do you have related to your data? (at least 2)