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What would you expect to observe if you were able to “transplant” CreS into a rod-shaped bacterium such as Bacillus subtilis?
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- When counting bacteria, the difference between the standard plate (STP) count and a direct microscopic count is that STP counts the number of ......... ...cells only.When do we make the determination that a bacterium is Gram positive? A) Safranin washes the crystal violet die out of the cell wall. B) Crystal violet die is not able to absorb into the cell wall. C) Alcohol does not wash the crystal violet die out of the cell wall. D) Crystal violet turns the bacterium a yellow color.What is the reason why we need to know if the bacteria is encapsulated or not?
- I am doing my microbiology homework and I need help with these questions: 1) List the structures ALL bacteria possess. 2) Identify three structures SOME but not all bacteria possess. 5) Describe the structure and function of three different structures found outside of the bacterial cytoplasmic membrane. 6) Differentiate between the two main types of bacterial envelope structures. 7) Why are Gram-positive cell walls stronger than Gram-negative cell walls? 8) Name a substance in the envelope of SOME bacteria that can cause severe symptoms in humans. 9) Describe the causes of sporogenesis and germination 10) Compare and contrast the major features of archaea, bacteria and eukaryotes by completing the table below. Characteristic Bacteria Archaea Eukarya Chromosome Type of Ribosomes Protein Synthesis Similar to Eukarya Sterols In Membrane Membrane-bound Organelles Peptidoglycan in Cell wallCiting data from the results table, tell which antibiotic is most effective against this type of bacterium. Citing data from the results table, which antibiotic if least effective against this type of bacterium. Explain how you determined the level of effectiveness. Describe what that Petri dish looked like. Hint(a most effective antibiotic has a larger clear area around the antibiotic disk. (a least effective antibiotic has a smaller clear area around antibiotic disk.)Why is aseptic urine collection important when cultures are ordered? If you counted 20 colonies from a 0.01-ml inoculum of a 1:10 dilution of urine, how many organisms per milliliter of specimen would you report? Is this number significant? What can you learn from visual inspection of a urine specimen? How would you relate these to the microorganisms present in the sample? How are UTIs acquired/transmitted? Explain why E. coli is frequently implicated in cystitis in females.
- 14) A streptobacillus shaped bacteria have the following shape/sructure: a) long chain of capsule/rodshaped cells b) long chain of spherical-shaped cells c) a single spherical-shaped cell d) a single capsule/rod-shaped cell e) none of the above.15) If you are a passenger on the “Magic School Bus” and you want to “explore” the cytoplasmic component of a Gram -ve bacteria, you will have to go through: a) two membranes separated by periplasm and thick peptidoglycan region b) two membranes separated by periplasm and thin peptidoglycan region c) A glycocalyx and a membrane separated by a thin peptidoglycan region d) a thin peptidoglycan region & one membrane e) a thick peptidoglycan region & one membrane 16) If you are a passenger on the “Magic School Bus” and you want to “explore” the cytoplasmic component of a Gram +ve bacteria, you will have to go through: a) two membranes separated by periplasm and thick peptidoglycan region b) two membranes separated by periplasm and thin…draw the Structure of a bacterium.35) Researchers have found a new type of bacteria, and they want to determine its phylum. What would be the most reliable method to do so? A) Conducting a Gram-stain test B) Performing an analysis of metabolic pathways C) Determining the ribosomal RNA sequence of the bacteria D) Analyzing the morphological characteristics of the bacteria
- Why is it important to know if the bacteria is encapsulated or not?Bacterial growth depends on many environmental factors, including the temperature of the environment. Since microbes can survive in a certain range of temperatures and will thrive at a temperature, understanding these variables allows for control of their growth. This understanding can be used, for example, to preserve certain foods or to treat infections. This lab simulation will use an instrument called a spectrophotometer. This instrument quantitatively measures the amount of light that is absorbed or transmitted by molecules in solution a) In your own words give an introduction to the microbial growth and the effect tempature has. b) In your own words explain the importance of the spectrophotometer in regards to microbial growth. c) In your own words give a hypothesis on how bacteria will react to different temperatures.Note that it is not appropriate to self-diagnose outside of a medical context and this is a completely hypothetical scenario. Imagine you have a rash on your foot. You're concerned that it's an infection and inoculate a sample onto an agar plate. You wonder, How can I figure out whether the pathogen is a bacterium vs a eukaryote? You decide to use lab supplies to get a basic understanding of the pathogen. Be specific about what tests you use and what you expect the results to be. Limit yourself to experiments we could do in our lab. What is one experiment you could do, involving culturing the organism?