What may be the challenges that you face while trying to isolate a protein? How could you use those challenges in turn to unfold a protein?

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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What may be the challenges that you face while trying to isolate a protein? How could
you use those challenges in turn to unfold a protein? 

Expert Solution
Protein purification and isolation

Proteins are polymers of amino acids. Proteins form the structural and catalytic framework of a cell. They have diverse array of functions. In order to work out with Proteins and study its function, first they have to be isolated. Therefore, Protein isolation forms an important application. 

Proteins can be isolated by many techniques. The best and most commonly used are:

  • homogenization of sample
  • Protein precipitation and dialysis to remove the salt
  • Chromatography 
  • Electrophoresis 
  • Immunoprecipitation

 

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