What is/are the independent variable/s?What is the dependent variable?What is/are the control/s? 4 nurseCurionHy a traywe have the1 Obtain a total of 6 test tubes, label test tubes A, B, C. Do not label the other 3 test tubes.2 Add 2 ml of distilled water to test tube A. Gently swirl the test tube.at trst, hebosameProcedure 3Effects of pH on Enzyme Activityin a3 Add 2 ml of hydrochloric acid (HC) to test tube B. Gently swirl the test tube.* Add 2 ml of sodium hydroxide (NaOH) to test tube C. Gently swirl the test tube.5 Add 1 ml of stock enzyme solution to test tubes A, B, C.ác a small piece of pH paper and tweezers, tilt the test tube slightly to test the pH of test tube A, B and C (use a newpiece for each test tube), Record results.7 To each of the three unmarked tubes add 1 ml of hydrogen peroxide.O AT THE SAME TIME, pour the contents of the unmarked tubes into each of the labeled test tubes.9 Record your results. Note the relative rates of each individual reaction.Predict: Which pH do you think would be optimum for the catalase to function? Why or why not?What is the independent variable of this reaction?What is the dependent variable of this reaction?Part 3 Table-Effects of pH on Enzyme ActivitypHResultsTubeContentsbubbledDistilled water +A1 ml enzymeslightlybubbled3.HCI +1 ml enzymeBNaOH +12.5C1 ml enzymePart 3 Questions:1 What effect does a high pH (base), low pH (acid), and neutral pH have on the enzyme activity? Use evidence to supportyour answer.2 Which pH is optimal for this reaction to occur? How does this relate to what we know about the body's normal pHlevel? Use evidence to support your answer.CHAPTER 5 | Catalysts for Change Understanding EnzymesBiology 189

The dependent variable is the variable that is being measured. An independent variable is the…

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What is/are the independent variable/s? What is the dependent variable? What is/are the control/s?

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

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Chapter1: The Human Body: An Orientation

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Question

What is/are the independent variable/s? What is the dependent variable? What is/are the control/s?

4 nurse
CurionHy a tray
we have the
1 Obtain a total of 6 test tubes, label test tubes A, B, C. Do not label the other 3 test tubes.
2 Add 2 ml of distilled water to test tube A. Gently swirl the test tube.
at trst, he
bo
same
Procedure 3
Effects of pH on Enzyme Activity
in a
3 Add 2 ml of hydrochloric acid (HC) to test tube B. Gently swirl the test tube.
* Add 2 ml of sodium hydroxide (NaOH) to test tube C. Gently swirl the test tube.
5 Add 1 ml of stock enzyme solution to test tubes A, B, C.
ác a small piece of pH paper and tweezers, tilt the test tube slightly to test the pH of test tube A, B and C (use a new
piece for each test tube), Record results.
7 To each of the three unmarked tubes add 1 ml of hydrogen peroxide.
O AT THE SAME TIME, pour the contents of the unmarked tubes into each of the labeled test tubes.
9 Record your results. Note the relative rates of each individual reaction.
Predict: Which pH do you think would be optimum for the catalase to function? Why or why not?
What is the independent variable of this reaction?
What is the dependent variable of this reaction?
Part 3 Table-Effects of pH on Enzyme Activity
pH
Results
Tube
Contents
bubbled
Distilled water +
A
1 ml enzyme
slightly
bubbled
3.
HCI +
1 ml enzyme
B
NaOH +
12.5
C
1 ml enzyme
Part 3 Questions:
1 What effect does a high pH (base), low pH (acid), and neutral pH have on the enzyme activity? Use evidence to support
your answer.
2 Which pH is optimal for this reaction to occur? How does this relate to what we know about the body's normal pH
level? Use evidence to support your answer.
CHAPTER 5 | Catalysts for Change Understanding Enzymes
Biology 189

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