) What is the total net charge of double-stranded DNA that is 500 basepairs (bp) in length? b) In agarose gel electrophoresis, two parallel and oppositely charged electrodes are situated at opposite ends of the gel. The surrounding solution is a "buffer", an aqueous salt solution maintained at constant pH. The gel is made in the same buffer, with a small concentration of agarose. Because the concentration of agarose is small... everything between the electrodes can be considered to have the same dielectric constant. In the schematic below, sketch the electric field lines within the apparatus, taking care to note their direction. c) In what direction would the DNA feel a force and move spontaneously because of this electric field? And Generally, the user "runs a gel" by applying a constant (DC) voltage between the two electrodes. A common operating voltage is 90V. Under this potential difference, the current flowing between the two electrodes is measured to be 60 mA. What is the resistance of the gel + buffer solution between the electrodes? Show your work
) What is the total net charge of double-stranded DNA that is 500 basepairs (bp) in length? b) In agarose gel electrophoresis, two parallel and oppositely charged electrodes are situated at opposite ends of the gel. The surrounding solution is a "buffer", an aqueous salt solution maintained at constant pH. The gel is made in the same buffer, with a small concentration of agarose. Because the concentration of agarose is small... everything between the electrodes can be considered to have the same dielectric constant. In the schematic below, sketch the electric field lines within the apparatus, taking care to note their direction. c) In what direction would the DNA feel a force and move spontaneously because of this electric field? And Generally, the user "runs a gel" by applying a constant (DC) voltage between the two electrodes. A common operating voltage is 90V. Under this potential difference, the current flowing between the two electrodes is measured to be 60 mA. What is the resistance of the gel + buffer solution between the electrodes? Show your work
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answer a,b and c... Q24
Transcribed Image Text:a) What is the total net charge of double-stranded DNA that is 500 basepairs (bp) in
length? b) In agarose gel electrophoresis, two parallel and oppositely charged electrodes
are situated at opposite ends of the gel. The surrounding solution is a "buffer", an
aqueous salt solution maintained at constant pH. The gel is made in the same buffer,
with a small concentration of agarose. Because the concentration of agarose is small.
everything between the electrodes can be considered to have the same dielectric
constant. In the schematic below, sketch the electric field lines within the apparatus,
taking care to note their direction. c) In what direction would the DNA feel a force and
move spontaneously because of this electric field? And Generally, the user "runs a gel"
by applying a constant (DC) voltage between the two electrodes. A common operating
voltage is 90V. Under this potential difference, the current flowing between the two
electrodes is measured to be 60 mA. What is the resistance of the gel + buffer solution
between the electrodes? Show your work.
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