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What is a good strategy for isolating spore-forming
bacteria from an environmental sample?
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- WHAT IS THE GENERAL PROTOCOL WHEN ISOLATING A SPECIFIC BACTERIA FROM A MIXED ENVIRONMENTALSAMPLE WHICH INCLUDES SAMPLING, CULTURING, AND IDENTIFICATION OF THE BACTERIA?Describe the principle of MPN for enumerating bacteria from a natural sample.In zone electrophoresis, why is it possible to isolate the components of a sample and recover them for identification? a) what influences the direction of migration of the components of a sample in zone electrophoresis?
- Why is pure culture and culture collection important in identifying novel species of bacteria?Why might a microarray be superior to usinghigh-throughput sequencing to identify a rarepopulation member in a complex microbial community?What are the advantages and limitations of FISH andPhyloChips for analysis of microbial communities?What are the standard protocols in the isolation of archaea?
- The isolation of Salmonella Typhimurium and S.Typhi require different protocols: A) In what aspects do these protocols differ? B) why do these protocols differ? C) How will you be able to distinguish between these organisms on XLD and Hektoen Agar?Why are macroscopic methods such as motility agar and colonial morphologic examination useful for classifying bacteria?What is an example of an experimental approach for determining whether a beef burger contains a food poisoning bacterium?
- How do you recognize individual colonies? Describe color, shape, size. Explain how the streak plate method enabled you to identify a single species from a mixed culture?The process of collecting characteristics of your unknown bacterium can be helped by the use of a dichotomous key. A dichotomous key is a flowchart that can be used to identify an organism. a) in your own words explain how to create and use a dichotomous key to identify unknown bacteriaDescribe the enrichment strategy behind Beijerinck’s isolation ofAzotobacter.