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- Pls Help me. 2. If a selection assay be made to identify cells that have incorporated the recombinant DNA, what type of medium will be used? How will it work?Please describe the detailed procedures of southern blot. Explain the protocol and principle of restriction fragment length polymorphism (RFLP) and how RFLP is used for genetic disease diagnosis.Why is DNA microarray an important tool and explain How is DNA microarray used in detecting cancer?
- In ELISPOT Assay, explain the importance of adding stimulus to the 96-well plate during the incubation of Peripheral Blood mononuclear cellsChoose two genes from Figure 4.6 and draw a graph to represent the change in transcription over time.The strain of λ phage t is cI857. That tells you that the cI DNA segment is disabled by a specific mutation. What is the exact genetic change in cI857? What specific property of the cI gene product does this mutation change, and how does this help titering for a plaque assay?
- I understand that microarrays are being used to define the molecular abnormality and the prognosis in some patients with leukaemia. What are microarrays?Breast Cancer Screening Program – A group of women were subjected to a breast cancer screening program. A newly developed screening test was utilized to measure its sensitivity and specificity against a gold standard test. a) What is the prevalence of breast cancer in this population? b) Calculate the Sensitivity of the test and provide interpretation c) Calculate the Specificity of the test and provide interpretationExplain how DNA microarrays are used in molecular profiling ofcancerous tumors.
- In the plaque assay, exactly what makes up a single plaque?Explain The replica plating technique?Fluorescence in-situ hybridisation (FISH) analysis can be performed on fixed pathological tumour sections. Briefly outline why interphase FISH is used on fixed material from a solid tumour, rather than metaphase FISH