Q: Describe how the streak-plate method is used to obtain a pure culture and how the resulting culture…
A: In microbiology, culture refers to microbial growth of a certain species maintained in controlled…
Q: Why does a streaking method used to inoculate plates result in isolated colonies?
A: Streaking method is used to obtain pure colonies of microbes(bacteria) from a proper single species…
Q: What is agar and why is it necessary for growing bacteria on plates?
A: Agar is a mixture of two polysaccharides- agarose and agaropectin. It is derived from red-purple…
Q: What is the difference between culture-based technique and culture-independent techniques based on…
A: A microbiological culture, also known as a microbial culture, is a way of multiplying microbial…
Q: What are the disadvantages of doing a total microbial count by examining wet mounts under the…
A: Ans- Wet mounts have the following drawbacks: The benefit of being able to observe movement can also…
Q: Why is it important for a microbiology laboratory technician to use aseptic technique?
A: The majority of the microbes onto which scientists work in laboratories are known pathogens.
Q: Why is it necessary to maintain aseptic conditions in microbiology?
A: Aseptic conditions means microbs free environment. Microbs means bacteria, virus and fungi. It may…
Q: What is the importance of agar plates and bacteria cultures in food microbiology?
A: There are different culturing techniques such as agar plates, broth culture, stab culture and solid…
Q: How does bacteria grow on agar plates?
A: Bacteria are the most primitive organisms on Earth. They can survive in most extreme environments as…
Q: What is the primary use of agar deep, agar slant, agar plate? explain
A: Primary use of agar deep : • Agar deeps are used to grow bacteria which require less oxygen . And…
Q: What are the 4 types of culture?
A: Biological cultures include common laboratory means, which are involved in studying living organisms…
Q: Why is it important to become familiar with the microbiology laboratory's apparatus and equipment?
A: You should always know your lab equipment well before you do any type of experiment because without…
Q: What are the equipments/ materials used in imoobilizing and separating cells when using: spread…
A: Aseptic techniques refer to any method which is used to sterilize and maintain sterility of the work…
Q: Why are semisolid media sometimes preferred over slide techniques for evaluating bacterial motility?
A: Semi-solid media are soft media that is used in demonstrating the motility of bacteria and…
Q: What are the advantages and disadvantages of observ-ing living-specimen slides and…
A: Microorganisms are the organisms that are not visible to the naked eye. Their size ranges in few…
Q: Describe the step by step method of preparing culture media in microbiology.
A: Culture media are the media or the substrate used to grow microorganisms. There are different types…
Q: what is the culture media for gram-positive bacteria?
A: The culture media for gram positive bacteria is Mannitol salt agar . The presence of bile salts, as…
Q: Why might viable cell cultures be of more use in microbialtaxonomy than preserved specimens?
A: Microbial taxonomy is a field of biological sciences that deals with the grouping of microorganisms…
Q: Describe and give the principle of each equipment used for sterilization in microbiology.
A: Sterilization is the method of removal, killing or inhibition of all forms of lives, spores or…
Q: Why did we both stab and streak our blood agar plates when inoculating them with bacteria?
A: Blood agar is a nutrient medium which contain essential nutrients for bacterial growth and blood of…
Q: What is the basis of enrichment culture techniques? Why is an enrichment medium usually suitable for…
A: Enrichment culture media is the one which is used for the growth and isolation of a specific type of…
Q: How is air contamination prevented when an inoculating loop is used to introduce or take a bacterial…
A: Biotechnology is a branch of biology, including the use of living organisms to produce products.…
Q: What is Bacterial Injury in Microbiology?
A: Bacteria produced in pure culture under ideal circumstances are examined in the lab for their…
Q: What is the main advantage of using the slide culture technique for identifying molds?
A: Side Culture - It is a rapid method of preparing fungal colonies for examination and identification.…
Q: Can simply observing the colonies formed on agar plates and the results of a few biochemical tests…
A: These are biochemical test is to perform to identify different type of bacteria.
Q: Distinguish batch culture and continuous culture
A: Fermentation is used to convert carbohydrates into alcohol or acids. This process occurs in some…
Q: How can moist heat sterilization can sterilize antibiotic solution?
A: Moist heat sterilization is a technique that uses heated, increased pressure steam to sterilize an…
Q: What other fields of science besides microbiology is the aseptic technique used?
A: Aseptic technique refers to the procedures that prevent the contamination of microorganisms. They…
Q: List the step-by-step procedure of Gram staining.
A: Gram staining, established by Danish Microbiologist Hans Christian Gram in 1884, is by far the most…
Q: How does the agar dilution method differ from streaking toobtain isolated colonies?
A: Both these are methods to culture microorganisms and have their respective pro and cons as mentioned…
Q: What is the nature of samples that should NOT be placed inside the drying-oven?
A: According to the question, we have to explain the nature of the samples that should not be placed…
Q: What could be possible errors when you incorrectly identify an Unknown organism when using IMViC…
A: IMViC tests are used to identify bacteria from specimens collected from patients in majority of…
Q: How do we isolate culture media (step by step process) and what is the purpose of bacterial culture…
A: Introduction :- Microorganisms can be found in the natural environment, such as soil. They coexist…
Q: what are the reagents for the four major steps of the gram stain procedure
A: Gram staining protocol is used to differentiate between Gram-positive and Gram-negative bacteria by…
Q: What are the advantanges and disadvantages of using stained preparations for observing microbial…
A: Characteristics of microbes are studied using a microscope by microbiologists. Some microbes like…
Q: selective agar media for gram-positive bacteria?
A: Phenyl Ethyl Alcohol Blood Agar is a selective media for gram positive bacteria.
Q: How do you do the continuous culture of bacteria by chemostat and turbidostat
A: Answer: CONTINUOUS CULTURE : It is the method of culturing/growing of bacteria in a media in a…
Q: Turbidometry is a method used for counting microbes?
A: Microbiology is the branch of biology that deals with microorganisms. Bacterial cultures and their…
Q: Describe the difference between batch and continuous culture techniques
A: Bacteria and fungi, for example, are extremely valuable to a wide range of industries.…
Q: What are the different Microbiological sterilization techniques used for Microbiology equipment and…
A: Microbiology is the branch of biology that deals with the study of microorganisms such as bacteria,…
Q: Briefly, in your own words, describe the steps of the gram staining procedure.
A: The bacterial kingdom is classified into Gram positive and Gram negative bacteria depending on their…
Q: How to prepare an agar plate for bacteria? How to include the antibiotics into agar plates? How long…
A:
Q: What method can be used to achieve sterilization?
A: It is required to describe the method to be used to achieve sterilization.
Q: What is this media? What type of media is it? Which type of organism can grow on this plate?…
A: Media is... Agar This is the solids type of Media. Organism type... Streak plate technique is used…
Q: What type of reaction or lab test differentiates the presence of either staphylococcus bacteria or…
A: Staphylococcus Bacteria which grows in cluster are Gram-positive (catalase-positive). Streptococcus…
Q: What is the goal of isolation streak plate technique? Why is microbial considered a pure culture?
A: Streaking : Streaking for isolation on an agar plate involves the successive dilution of organisms…
What are the purposes of carrying out the streak plate and
pour plate techniques in
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- What are the advantages and disadvantages of culture-dependent and culture-independent methods in microbiology, respectively.What are the consequences if aseptic techniques are not strictly followed in performing microbiology experiments?What is the difference between culture-based technique and culture-independent techniques based on the principle behind the method?
- How do we isolate culture media (step by step process) and what is the purpose of bacterial culture methods?What are the advantages and limitations of studying bacteria by means of the Culture method?What is an E-test strip, and what is it used for in the microbiology laboratory? Draw a diagram of an E-test strip.
- What is the basis of enrichment culture techniques? Why is an enrichment medium usually suitable for the enrichment of only a certain group or groups of organisms?What other fields of science besides microbiology is the aseptic technique used?What could be possible errors when you incorrectly identify an Unknown organism when using IMViC tests in Microbiology?