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What are some of the strength and limitation of culture-independent methods in the study of microbial ecology?
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- What is the difference between culture media and cultural environment? Are environmental conditions related to specific microbes? If so, which conditions for which type of microbes? If you were a field researcher collecting microbes in the wild, what kind of data would you take to make sure you know the exact environment the samples require in order to culture them in a lab?In an in situ sample, describe the method that you woulduse to study the symbiotic interactions between various microbial species?What are the physical requirements that must be considered when trying to grow microbes?
- What are the advantages and disadvantages of culture-dependent and culture-independent methods in microbiology, respectively.What are the advantages and disadvantages of plate count technique over other methods of quantifying microbial populations?Explain the role of cultivation-independent methods to study microorganisms in the environment.
- Enumerate 15 genus/species of bacteria that thrives on a rare microbial biosphere and provide the following details for each genus/species (prepare in table form): a. General characteristics b. Metabolic diversity (e.g. chemoorganotrophs, photoautotrophs, etc.) c. Most suitable methodological assessment (e.g. OTUs, ASVs, Metagenome Sequencing, etc.)What are the consequences if aseptic techniques are not strictly followed in performing microbiology experiments?how did winogradsky and beijerink contribute to the study of microbial ecology ? what new culturing techniques did they develop in their studies