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What advantage does the oil immersion objective have over the low power objective when doing bacterial examination?
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- What are the differences between scanner, low power objective, high power objective and oil immersion objective? Introduce the terms stated above and explain comprehensively.What are the advantages and disadvantages of using the Wet Mount technique? What are the advantages and disadvantages of using the Hanging drop technique? What are the advantages and disadvantages of using the Slide Culture technique? pls elaborate each, thank youHow do eosin-methylene blue (EMB) agar plates work? What organism(s) are they designed to detect? How would a positive test appear on the plate?
- What is the purpose of examining your specimen slide using low power objective first nefore high power objective?if an unknown bacterial slide appears to be in fading color and the shape of the specimen seems to be diffused. what troubleshooting of the focusing is needed in order to see the bacteria clearly?The first step in identifying an organism from a pure working culture is to perform a(n) __________ test. acid-fast staining negative staining endospore staining simple staining Gram stain
- You are hired in one of the microbiology labs in Memphis and your first assignment to identify microbial samples from a local restaurant. You are given a set of slides and asked to classify the specimens as Gram positive or Gram negative. Based on this information answer questions 1-10:1) Which objective lens you should use first and what is the total magnification?2) Which focus knob you must use in adjusting the focal point using the above lens?3) What is the highest magnification and what would be the total magnification?Answer the following questions: 1. Why do we pass the smear in the open flame of the bunsen burner 3 times? What do we want to achieve by doing this? 2. Is the size of the bacterial smear on the slide very critical to the analysis? Why or why not? 3. Is the thickness of the bacterial smear on the slide very critical to the analysis? Why or why not? 4. Why do we pass the smear in a gentle stream of water after flooding it with a stain? What do we want to prevent by doing this? 5. Is the duration or time of stain application very critical in simple staining? Why or why not? NOTE: Please try to answer all of the questions asked, i promise to give you a good ratingswhy is it necessary to use oil immersion on oil objective ?
- Why are thick or dense smears less likely to provide a good smear preparation for microscopic evaluation?What are the disadvantages of doing a total microbial count by examining wet mounts under the microscope?In preparing a bacterial smear for staining, heat fixation is done after the smear dries up.a.) Give the purpose of heat fixation.b.) What can be observed in wet mounts or hanging-drop slides that cannot be observed in heat-fixed slides?