What advantage do fluorescent dyes and fluorescence microscopy provide in comparison to the chemical dyes used to stain specimens for light microscopy?

Q: Explain when to use bright-field, phase-contrast, dark-field, fluorescence, transmission electron,…

A: There are different types of microscopy like bright-field, phase-contrast, dark-field, fluorescence,…

Q: Write the steps involved in simple staining ? And what the purpose of doing it ?

A: It is a conventional staining technique. The method only uses a single stain and is a direct…

Q: can you discuss the best practices to obtain an image of good quality in microscope?

A: Obtaining high-quality microscope images is essential for accurate scientific research and analysis.…

Q: What factors must be addressed to properly acquire quantitative fluorescence microscopy images? What…

A: Quantitative fluorescence microscopy is a type of microscopy used in biological studies in which the…

Q: What is the the total magnification of a microscope that is set up using a 10x ocular lens and a 20x…

A: A microscope is a scientific tool that is used to look at organisms or things that can't be seen…

Q: Why should you always begin to use a microscope with a low-power objective?

A: Microscope is an instrument used to see the objects that cannot be seen by naked eye. A light…

Q: a) Briefly discuss the limits of resolution in optical microscopy?      b) What is empty…

A: An equipment in the lab used to study items too tiny for the human eye is a microscope. The…

Q: What are the advantages and disadvantages of gram staining

A: Introduction  Gram staining is a widely used laboratory technique in microbiology to differentiate…

Q: Define field of view and working distance as it relates to working with a microscope.

A: Introduction  A microscope is a scientific tool that is used to investigate substances that are too…

Q: Can colorless samples be analyzed using a UV-Vis spectrophotometer? Yes or No or Maybe?

A: UV-Vis Spectroscopy  It is defined as a quantitative technique that is used to identify the…

Q: Calculate the diameter of the field of view for each total magnification on your microscope in…

A: Field of view (FOV) is the diameter of the circle of light that can be seen while looking into the…

Q: If accessories for darkfield microscopy were not available, how would you construct a simple star…

A: The microscope is an instrument used to view small objects that cannot be seen with an unaided eye.…

Q: Why is wavelenth the main limiting factor on limit of resoltuin in light microscopy?

A: NOTE: AS PER OUR GUIDELINES WE ARE SUPPOSED TO ANSWER ONLY FIRST QUESTION, PLEASE RESUBMIT THE OTHER…

Q: what is the source of illumination for a light microscope?

A: A microscope is an optical instrument used to view objects, for example, animal or plant cell, that…

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.