We have assayed LDH activity in a direction by following the reduction of lactate and NAD+ to pyruvate and NADH (as shown below). lactate + NAD+ pyruvate + NADH NADH absorbs maximally at 340nm; hence we can monitor LDH activity using AA340/min data. This reaction is also reversible and in fact, LDH prefers converting pyruvate to lactate. Is it possible to assay LDH activity in a reaction cocktail containing pyruvate and NADH? If yes, please describe shortly how would modify your data collection method on the spectrometer and what would you expect your AA340/min data to be like?
We have assayed LDH activity in a direction by following the reduction of lactate and NAD+ to pyruvate and NADH (as shown below). lactate + NAD+ pyruvate + NADH NADH absorbs maximally at 340nm; hence we can monitor LDH activity using AA340/min data. This reaction is also reversible and in fact, LDH prefers converting pyruvate to lactate. Is it possible to assay LDH activity in a reaction cocktail containing pyruvate and NADH? If yes, please describe shortly how would modify your data collection method on the spectrometer and what would you expect your AA340/min data to be like?
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Transcribed Image Text:We have assayed LDH activity in a direction by
following the reduction of lactate and NAD* to
pyruvate and NADH (as shown below).
lactate + NAD+
pyruvate + NADH
NADH absorbs maximally at 340nm; hence we can
monitor LDH activity using AA340/min data.
This reaction is also reversible and in fact, LDH
prefers converting pyruvate to lactate.
Is it possible to assay LDH activity in a reaction
cocktail containing pyruvate and NADH?
If yes, please describe shortly how would modify
your data collection method on the spectrometer
and what would you expect your AA340/min data to
be like?
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