w sample with a microscope: Focus on cells along the edge of the leaf: Use the 4X objective E, then the 10X and the 40X objective. Remember to use ONLY the fine focus when on the 10X 140X objectives. Make notes about the distribution of the chloroplasts in the cells - are they nly scattered inside the cells? Are they pushed to the edges of the cells? Are they moving (the wement might be very slow)? p the slide in this position. Place a drop of the 10% NaCl solution on the right edge of the erslip. While viewing the cells in the microscope, touch a dry Kimwipe to the left edge of the erslip. Capillary action will draw the NaCl solution over the Elodea leaf. You may have to adjust Fine focus of the microscope if the cells become blurry, as this is happening. Elodea cells in tap water (400) Bacteria pdf chloroplasts vacuole Elodea cells in 10 % NaCl (400 X).

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### Elodea Leaf Microscope Activity

**Step-by-Step Guide**

1. **Prepare a Wet Mount Slide**
   - Select a green, healthy leaf from an Elodea plant.
   - Place the leaf on a clean slide.
   - Add a drop of water on top of the leaf.
   - Cover it with a coverslip.

2. **View Sample with a Microscope**
   - Focus on cells along the edge of the leaf.
   - Start with the 4X objective first, then shift to the 10X and 40X objectives.
   - Use ONLY the fine focus when using the 10X and 40X objectives.
   - Note the distribution of chloroplasts in the cells:
     - Are they evenly scattered inside the cells?
     - Are they pushed to the edges of the cells?
     - Are they moving (the movement may be very slow)?

3. **Introduce NaCl Solution**
   - Maintain the slide in this position.
   - Place a drop of 10% NaCl solution on the right edge of the coverslip.
   - While observing the cells under the microscope, touch a dry Kimwipe to the left edge of the coverslip.
   - Capillary action will draw the NaCl solution over the Elodea leaf.
   - Adjust the fine focus if the cells become blurry during this process.

**Visual Aid: Microscope Images**
- **Image 1:** Elodea cells in tap water (400X magnification)
  - Clearly display chloroplasts and vacuoles.
- **Image 2:** Elodea cells in 10% NaCl (400X magnification)
  - Observe the changes in cell structure and chloroplast distribution due to the introduction of the NaCl solution.

These steps facilitate the observation of how Elodea cells react to changes in their environment, emphasizing the importance of the microscopic structure in biological studies.
Transcribed Image Text:### Elodea Leaf Microscope Activity **Step-by-Step Guide** 1. **Prepare a Wet Mount Slide** - Select a green, healthy leaf from an Elodea plant. - Place the leaf on a clean slide. - Add a drop of water on top of the leaf. - Cover it with a coverslip. 2. **View Sample with a Microscope** - Focus on cells along the edge of the leaf. - Start with the 4X objective first, then shift to the 10X and 40X objectives. - Use ONLY the fine focus when using the 10X and 40X objectives. - Note the distribution of chloroplasts in the cells: - Are they evenly scattered inside the cells? - Are they pushed to the edges of the cells? - Are they moving (the movement may be very slow)? 3. **Introduce NaCl Solution** - Maintain the slide in this position. - Place a drop of 10% NaCl solution on the right edge of the coverslip. - While observing the cells under the microscope, touch a dry Kimwipe to the left edge of the coverslip. - Capillary action will draw the NaCl solution over the Elodea leaf. - Adjust the fine focus if the cells become blurry during this process. **Visual Aid: Microscope Images** - **Image 1:** Elodea cells in tap water (400X magnification) - Clearly display chloroplasts and vacuoles. - **Image 2:** Elodea cells in 10% NaCl (400X magnification) - Observe the changes in cell structure and chloroplast distribution due to the introduction of the NaCl solution. These steps facilitate the observation of how Elodea cells react to changes in their environment, emphasizing the importance of the microscopic structure in biological studies.
### Lab Activity and Analysis: Osmosis in Elodea Cells

#### Consider your results from Part II Section D of the lab protocol and answer the following questions:

**a. Explain why the Elodea cells shrivel when placed in a 10% NaCl solution.**

**b. Explain in terms of the net movement of water across the plasma membrane. Why did they not rupture?**

**c. Explain why the Elodea cells returned to their typical structure when placed back in distilled water.**

**d. Explain in terms of the net movement of water across the plasma membrane.**

---

**Analysis and Discussion:**

**Question a:**
- Elodea cells shrivel in a 10% NaCl solution due to osmosis. The surrounding solution is hypertonic relative to the cell's cytoplasm, causing water to move out of the cells, leading to shriveling.

**Question b:**
- The shrinking or plasmolysis occurs because water moves out of the cell, but the cells do not rupture due to the flexible plasma membrane and cell wall that can maintain integrity and structure under osmotic pressure changes.

**Question c:**
- When Elodea cells are placed back in distilled water, which is hypotonic relative to the cytoplasm, water moves back into the cells, restoring them to their typical structure through the process of osmosis.

**Question d:**
- The net movement of water across the plasma membrane depends on the osmotic gradients formed by solute concentration differences. In distilled water, water moves into the cell to equalize solute concentration, resulting in the cell returning to its normal turgid state.

This section emphasizes the principles of osmosis, which are crucial in understanding cellular functions and responses to different environmental conditions. Familiarity with these concepts can aid in comprehending broader biological processes involving cellular transport and homeostasis.

---

This transcription provides a clear summary of the questions and a detailed explanation of the scientific concepts and processes involved, suitable for an educational website.
Transcribed Image Text:### Lab Activity and Analysis: Osmosis in Elodea Cells #### Consider your results from Part II Section D of the lab protocol and answer the following questions: **a. Explain why the Elodea cells shrivel when placed in a 10% NaCl solution.** **b. Explain in terms of the net movement of water across the plasma membrane. Why did they not rupture?** **c. Explain why the Elodea cells returned to their typical structure when placed back in distilled water.** **d. Explain in terms of the net movement of water across the plasma membrane.** --- **Analysis and Discussion:** **Question a:** - Elodea cells shrivel in a 10% NaCl solution due to osmosis. The surrounding solution is hypertonic relative to the cell's cytoplasm, causing water to move out of the cells, leading to shriveling. **Question b:** - The shrinking or plasmolysis occurs because water moves out of the cell, but the cells do not rupture due to the flexible plasma membrane and cell wall that can maintain integrity and structure under osmotic pressure changes. **Question c:** - When Elodea cells are placed back in distilled water, which is hypotonic relative to the cytoplasm, water moves back into the cells, restoring them to their typical structure through the process of osmosis. **Question d:** - The net movement of water across the plasma membrane depends on the osmotic gradients formed by solute concentration differences. In distilled water, water moves into the cell to equalize solute concentration, resulting in the cell returning to its normal turgid state. This section emphasizes the principles of osmosis, which are crucial in understanding cellular functions and responses to different environmental conditions. Familiarity with these concepts can aid in comprehending broader biological processes involving cellular transport and homeostasis. --- This transcription provides a clear summary of the questions and a detailed explanation of the scientific concepts and processes involved, suitable for an educational website.
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