Using the information provided, you must successfully calculate all the components of each of the three solutions listed below: Formula weights and form of reagents to be used for making the solutions below: - - 58.4 g/mol, comes as a solid 80 g/mol, comes as a solid sodium chloride ammonium nitrate potassium permanganate Tris Triton SDS BSA - 1 121.1 g/mol, comes as a solid 625 g/mol, comes as a liquid (100%) 228.4 g/mol, comes as a solid too big to list, comes as a solid Lysozyme too big to list, comes as a solid PEG (high mol weight) ~6000 g/mol, comes as a liquid (15% solution) B-ME 78.1 g/mol, comes as a liquid (55mM solution) - - - - - - 158 g/mol, comes as a solid 58.4 g/mol 2284 Solution 1: 50 mM sodium chloride, 0.5% SDS, 5 mg/ml BSA, 5 mM B-ME solid 7811 If you need 330 mL of solution 1, how much of each component would you need?

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**Solution Preparation Guide**

Using the information provided, you must successfully calculate all the components of each of the three solutions listed below.

**Formula weights and form of reagents to be used for making the solutions:**

- **Sodium chloride** – 58.4 g/mol, comes as a solid
- **Ammonium nitrate** – 80 g/mol, comes as a solid
- **Potassium permanganate** – 158 g/mol, comes as a solid
- **Tris** – 121.1 g/mol, comes as a solid
- **Triton** – 625 g/mol, comes as a liquid (100%)
- **SDS** – 228.4 g/mol, comes as a solid
- **BSA** – Too big to list, comes as a solid
- **Lysozyme** – Too big to list, comes as a solid
- **PEG (high mol weight)** – ~6000 g/mol, comes as a liquid (15% solution)
- **B-ME** – 781 g/mol, comes as a liquid (55 mM solution)

---

**Solution 1:**

**Components and Concentrations:**

- 50 mM sodium chloride (NaCl)
- 0.5% SDS
- 5 mg/ml BSA
- 5 mM B-ME

**Calculation Task:**

If you need 330 mL of Solution 1, how much of each component would you need?

Notes in the text:
- **NaCl:** 58.4 g/mol
- **SDS:** 228.4 g/mol, noted as solid
- **BSA:** noted as solid
- **B-ME:** 781 g/mol, noted as a liquid

Use these specifications to calculate the amounts based on the desired concentrations and solution volume.
Transcribed Image Text:**Solution Preparation Guide** Using the information provided, you must successfully calculate all the components of each of the three solutions listed below. **Formula weights and form of reagents to be used for making the solutions:** - **Sodium chloride** – 58.4 g/mol, comes as a solid - **Ammonium nitrate** – 80 g/mol, comes as a solid - **Potassium permanganate** – 158 g/mol, comes as a solid - **Tris** – 121.1 g/mol, comes as a solid - **Triton** – 625 g/mol, comes as a liquid (100%) - **SDS** – 228.4 g/mol, comes as a solid - **BSA** – Too big to list, comes as a solid - **Lysozyme** – Too big to list, comes as a solid - **PEG (high mol weight)** – ~6000 g/mol, comes as a liquid (15% solution) - **B-ME** – 781 g/mol, comes as a liquid (55 mM solution) --- **Solution 1:** **Components and Concentrations:** - 50 mM sodium chloride (NaCl) - 0.5% SDS - 5 mg/ml BSA - 5 mM B-ME **Calculation Task:** If you need 330 mL of Solution 1, how much of each component would you need? Notes in the text: - **NaCl:** 58.4 g/mol - **SDS:** 228.4 g/mol, noted as solid - **BSA:** noted as solid - **B-ME:** 781 g/mol, noted as a liquid Use these specifications to calculate the amounts based on the desired concentrations and solution volume.
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