Using the 'ABC model' of floral development, what would you expect the flowers of a mutant plant to look like if both A & B gene function had been destroyed? Edit View Insert Format Tools Table 12pt v Paragraph v BIU To
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- You are a developmental geneticist and perform a mutagenesis screen in Arabidopsis looking for floral organ mutants. You identify a mutant phenotype that has the following organ arrangement, beginning in whorl 1: Sepal-Sepal-Carpel-Carpel Which class of floral identity genes has been mutated? A-B double mutant O A O SEPALLATA B сThe ABCDE Model of flower development dictates the combination of transcription factors require to initiate each whorl of a flower. Based on the diagram below, what would happen if: Carpels and ovules Sepal Petal Stamen 2 4 Whorl A Genes E 25-40 The ABCDE model of floral organ determination in Arabidopsis In addition to the A-, B-, and C-function genes of the ABC model, this model includes two additional gene classes, D and E. In the ABCDE model, class A + E genes specify sepals; class A + B + E, petals; class B +C +E, stamens; class C + E, carpels; and class C+ D + E, ovules. 1. Gene D was non-functional: 2. Gene E was non-functional: 3. Only Gene A was functional: 4. Gene B and E were non-functional: 5. Only Gene A and Gene E were functional: Hint: If Gene A was non-functional the flower would have no sepals or petals, it would be composed of only stamens and carpels.Can you clearly type the answers to the all the parts to this question please a) What is the role of the LEAFY gene in floral development? (b) What is the phenotype of the leafy mutant? (c) Most of the ABC program genes are directly regulated by the transcription factor LEAFY (LFY). Still, LFY is expressed broadly throughout the floral meristem when the discrete A, B, and C domains are established. Discuss how this single broadly expressed transcription factor can generate distinct domains of ABC gene expression. Feel free to use diagrams.
- TOPIC: GENE INTERACTION AND EPISTASIS Two pure-breeding (homozygous) lines of shrubs were crossed as follows: P1 phenotype red leaves X green leaves P1 genotype ___________ _____________ F1 phenotype 100% green leaves with white flecks F1 genotype ___________ Crossing the F1s produced: F2 phenotypes F2 genotypes 123 green leaves w/ white flecks ____ 44 green leaves ____ 63 red leaves ____Pick an organelle or cellular structure that is unique to plants. Explain why this would be necessary for plant survival AND why it isn't necessary in animal cells. For the toolbar, press ALT+F10 (PC) or ALT+FN+F10 (Mac). B I U S Paragraph Arial 10pt A V x X, P QUESTION 21 Double fertilization involves 2 fertilizations, write the cells/nuclei involved with each, their ploidy level (ie. n, n+n, 2n, 3n). with a ploidy level of with a ploidy level of Fertilization 1: ,with a ploidy level of with a ploidy level of with a ploidy level of Fertilization 2: with a ploidy level ofstudent.masteryconnect.com Biology 1 Assessment 3 26 of 40 21 22 23 24 25 26 27 28 29 30 Finish Miller, Timia Polyploid plants form as a result of a beneficial mutation that often makes them larger and stronger than diploid plants. These plants form when a complete set of chromosomes fails to separate during meiosis creating plants with extra chromosomes. What type of mutation has this result? O frameshift O nondisjunction substitution O translocation esc 4. 5n
- Using the 'ABC model' of floral development, what would you expect the flowers of a mutant plant to look like if both A & B gene function had been destroyed?This is an image of a lily flower. Based on your reading, please click the part of the flower where pollen is deposited for fertilization. O Targets placed: 0/1 You can place up to 1 targets Undo Delete selected Remove Alld. Based on ABC model of floral development, this/these gene/genes controls/control the development of stamens in the third whorl of a flower. (A) A class gene (B) B + C class genes (C) A + B class genes (D) C class gene
- If you engineer an A::B construct into a B loss of function mutant Arabidopsis plant you would expect petals on each flower.What are homeotic genes? Please exaplain ow they control the sequence of whorl development in flowers according to the ABCDE model.EXPERIMENT 4: INDUCTION OF CALLUS SOMATIC EMBRYOGENESIS OF HAPLOID PLANTS Objective: To prepare anther as a source of explant To induce callus somatic embryogenesis of haploid plants Procedure: Cut off the buds and sort them into 3 developmental stages based on length of buds Surface sterilize the buds in 70% ethanol for 2-3 minutes, making sure the entire bud is immersed in the alcohol. Drain off excess alcohol from the buds and aseptically excise the anthers from each bud. Remove the filaments and culture the anther on one of the agar media provided. Label the stage of development of the bud on each petri dish as the anthers are cultured. Seal the Petri dishes with parafilm. Incubate in the dark at 26 to 28ºC for 4 to 8 weeks or until small plants can be seen growing out of the anthers. Then transfer to diffuse light. Record the results of the experiment in table form and submit the report. Observation: The culture was contaminated after 8 weeks of incubation…