Using the AACT method, calculate and compare the relative quantity value for your gene of interest and control using the below C₁ data. (control: control flies; test: experimental flies; tub: housekeeping gene; hsp: hsp gene) Sample CT Value control tub 16.13 control tub 15.97 control tub 15.72 test tub 17.54 test tub 17.01 test tub 18.18 control hsp 26.69 control hsp 26.22 control hsp 26.25 test hsp 22.31 test hsp 22.25 22.35 test hsp

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Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.